Hepatic Lipid Accumulation/ Steatosis Assay Kit (BN00813)

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BN00813
  • Hepatic Lipid Accumulation/ Steatosis Assay Kit (BN00813)
  • Hepatic Lipid Accumulation/ Steatosis Assay Kit (BN00813)
  • Hepatic Lipid Accumulation/ Steatosis Assay Kit (BN00813)
  • Hepatic Lipid Accumulation/ Steatosis Assay Kit (BN00813)
  • Hepatic Lipid Accumulation/ Steatosis Assay Kit (BN00813)
€577

Description

ELISA Kit Technical ManualMSDS

Hepatic Lipid Accumulation/ Steatosis Assay Kit

Hepatic Steatosis, also known as fatty liver, is a disease characterized by an excessive accumulation of lipid droplets within the liver. This can be caused by multiple factors, including excessive alcohol intake, obesity, and other diseases with abnormal fat metabolism. Hepatic Steatosis is commonly used as a marker to determine the hepatotoxicity of drug candidates. Assay Genie's Hepatic Steatosis Kit provides reagents for measurement of intracellular triglyceride accumulation and staining reagents to visualize the lipid droplet formation in hepatocytes. The kit can be used to screen and evaluate steatosis risk of drug candidates and to study the mechanism of steatosis development. Chloroquine is included as a positive control for inducing steatosis in liver cells, such as HepG2. The kit provides enough reagents for 100 assays for triglyceride quantitation and staining reagents enough for two 96-well plates, two 6-well plates, or four 100 mm culture dishes.

Figure: (a) Triglyceride Standard Curve by colorimetric (b) and fluorometric assay; (c) Measurement of Triglyceride in untreated HepG2 cells and cells treated with 5, 25, or 50 µM Chloroquine. (d) Untreated HepG2 cells stained with Oil Red O and methyl green, a nuclear stain. (e) HepG2 cells treated with 25 µM Chloroquine for 3 days and stained with Oil Red O and methyl green.

Description Key Information

Product SKU

BN00813

Size

100 assays

Measurement

Absorbance (OD 570 nm) or Fluorescence (Ex/Em = 535/587 nm) and Microscopy

Reactivity

Mammalian

Application

Evaluate hepatic steatosis, quantify triglycerides, and visualize lipid droplet accumulationScreen drug candidates for steatotic risk

Description

Only Kit on the market to use a dual method to study Hepatic Steatosis: Quantifies Triglycerides (colorimetric/fluorometric) and visualizes lipid droplets by staining with Oil Red O. The kit provides enough reagents for 100 assays for triglyceride quantitation and staining reagents enough for two 96-well plates, two 6-well plates, or four 100 mm culture dishes. Easy and simple protocol. This kit can be used to screen and evaluate steatosis risk of drug candidates and to study the mechanism of steatosis development. Chloroquine is included as a positive control for inducing steatosis in liver cells, such as HepG2.

Buffers

  • Triglyceride Assay Buffer Triglyceride Probe (in DMSO)
  • Lipase Triglyceride Enzyme Mix Triglyceride Standard (1 mM)
  • Chloroquine PBS Formalin (10%)
  • Oil Red O Methyl Green

Storage

4°C

Shipping

Gel Pack

Guidelines

For Research Use Only! Not For Use in Humans.

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