Hexokinase (HK) Inhibitor Screening Kit (Colorimetric) (BN01045)

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SKU:
BN01045
€726

Description

ELISA Kit Technical ManualMSDS

Hexokinase (HK) Inhibitor Screening Kit (Colorimetric)

Hexokinases (HK) play an important role in glucose metabolism. Hexokinases phosphorylate glucose and generate glucose-6-phosphate for glycolysis. Hexokinases have four isoforms (HK-I, II, III and IV). HK-I, HK-II and HK-III have low Km, while HK-IV has 100 fold high Km. Hexokinase deficiency leads to severe human diseases such as X-linked muscular dystrophy and a rare autosomal recessive hemolytic anemia. On the other hand, increased hexokinase activity is detected in various tumors and is associated with metastasis. In Assay Genie's Hexokinase Inhibitor Screening kit, glucose is converted to glucose-6-phosphate by hexokinase; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm. In the presence of a Hexokinase inhibitor, the reaction is impeded/abolished resulting in decrease or total loss of absorbance. This assay kit can be used to screen/study/characterize the potential inhibitors of Hexokinase. The assay is simple, high-throughput adaptable and can be performed within 30 min.

Figure: Inhibition of Hexokinase activity by Hexokinase inhibitor (Bromopyruvic Acid). Assay was performed following the kit protocol.

Key Information Description

Product SKU

BN01045

Size

100 assays

Detection Method

Absorbance at 450 nm

Applications

  • Screening/characterizing/studying potential inhibitors of Hexokinase.
  • A simple and high-throughput adaptable assay kit used to colorimetrically (450 nm) screen/study/characterize potential inhibitors of Hexokinase within 30 min.

Features and Benefits

  • Simple and High throughput adaptable
  • Colorimetric Detection
  • Screen within 30 min

Kit Components

  • HK Assay Buffer
  • HK Substrate
  • HK Coenzyme
  • HK Converter
  • HK Developer Hexokinase
  • HK Inhibitor Control

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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