Anti-HIST1H1D (Ab-85) Antibody (PACO60611)

Antibody Name:	HIST1H1D (Ab-85) Antibody (PACO60611)
Antibody SKU:	PACO60611
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, ChIP
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:10-1:100
Species Reactivity:	Human
Immunogen:	Peptide sequence around site of Lys (85) derived from Human Histone H1.3

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	Antigen Affinity Purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

IHC image of PACO60611 diluted at 1:20 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Chromatin Immunoprecipitation Hela (4*10⁶) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H1D (PACO60611) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta -Globin promoter.

Background:	Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Synonyms:	Histone H1.3 (Histone H1c) (Histone H1s-2), HIST1H1D, H1F3

UniProt Protein Function:	H1D: Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation. Belongs to the histone H1/H5 family.
UniProt Protein Details:	Protein type:DNA-binding Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: nuclear chromatin Molecular Function:chromatin DNA binding
NCBI Summary:	Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]
UniProt Code:	P16402
NCBI GenInfo Identifier:	121925
NCBI Gene ID:	3007
NCBI Accession:	P16402.2
UniProt Secondary Accession:	P16402,Q2M2I2, B2R751,
UniProt Related Accession:	P16402
Molecular Weight:	22,350 Da
NCBI Full Name:	Histone H1.3
NCBI Synonym Full Names:	histone cluster 1, H1d
NCBI Official Symbol:	HIST1H1D
NCBI Official Synonym Symbols:	H1D; H1.3; H1F3; H1s-2
NCBI Protein Information:	histone H1.3
UniProt Protein Name:	Histone H1.3
UniProt Synonym Protein Names:	Histone H1c; Histone H1s-2
UniProt Gene Name:	HIST1H1D
UniProt Entry Name:	H13_HUMAN

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)