Human ADAMTS20/A disintegrin and metalloproteinase with thrombospondin motifs 20 ELISA Kit
The Human ADAMTS20 (A Disintegrin and Metalloproteinase with Thrombospondin Motifs 20) ELISA Kit is a powerful tool for detecting and quantifying levels of ADAMTS20 in human samples. This kit is highly sensitive and specific, providing accurate and reproducible results for serum, plasma, and cell culture supernatants. ADAMTS20 is an important enzyme that plays a key role in a variety of biological processes, including extracellular matrix organization and tissue remodeling.
Dysregulation of ADAMTS20 has been linked to various diseases, making it a promising biomarker for research and clinical applications. By using the Human ADAMTS20 ELISA Kit, researchers can gain valuable insights into the role of ADAMTS20 in disease pathogenesis and progression. With its reliable performance and ease of use, this kit is an essential tool for studying ADAMTS20 and its implications in human health and disease.
Product Name:
Human ADAMTS20/A disintegrin and metalloproteinase with thrombospondin motifs 20 ELISA Kit
SKU:
HUFI01493
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
46.875 pg/mL
Range:
78.125-5000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
85-105%
85-105%
87-101%
EDTA Plasma (n = 5)
83-97%
82-95%
82-97%
Heparin Plasma (n = 5)
81-99%
80-96%
81-93%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
85-96
91
EDTA Plasma (n = 5)
87-104
95
Heparin Plasma (n = 5)
88-102
97
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
