Human TPA (311-562) Recombinant Protein (RPPB2336)
- SKU:
- RPPB2336
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P00750
- Research Area:
- Enzymes
Description
| Product Name: | Human TPA (311-562) Recombinant Protein |
| Product Code: | RPPB2336 |
| Size: | 10µg |
| Species: | Human |
| Target: | TPA (311-562) |
| Synonyms: | Tissue-type plasminogen activator, EC 3.4.21.68, tPA, t-PA, t-plasminogen activator, TPA, T-PA, DKFZp686I03148, PLAT and tPA. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | TPA protein solution (0.5mg/ml) contains 50mM MES (pH 5.0), 5mM CaCl2, 1mM DTT, 0.5M NaCl and 30% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | ADPIKGGLFA DIASHPWQAA IFAKHRRSPG ERFLCGGILI SSCWILSAAH CFQERFPPHH LTVILGRTYR VVPGEEEQKF EVEKYIVHKE FDDDTYDNDI ALLQLKSDSS RCAQESSVVR TVCLPPADLQ LPDWTECELS GYGKHEALSP FYSERLKEAH VRLYPSSRCT SQHLLNRTVT DNMLCAGDTR SGGPQANLHD ACQGDSGGPL VCLNDGRMTL VGIISWGLGC GQKDVPGVYT KVTNYLDWIR DNMRPHHHHH H |
Tissue plasminogen activator (abbreviated PLAT or tPA) is a secreted serine proteasewhich converts the proenzymeplasminogento plasmin, a fibrinolyticenzyme. Plasminogen is synthesized as a single chain which is cleaved by PLAT into the two chain disulfide linked plasmin.This enzyme plays a role in cell migrationand tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysiswhich can result in thrombosisor embolism.
TPA Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 261 amino acids (311-562 a.a.) and having a molecular mass of 29.2kDa (Migrates at 28-40kDa on SDS-PAGE under reducing conditions).TPA is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration. |
| NCBI Summary: | This gene encodes tissue-type plasminogen activator, a secreted serine protease that converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. The encoded preproprotein is proteolytically processed by plasmin or trypsin to generate heavy and light chains. These chains associate via disulfide linkages to form the heterodimeric enzyme. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding, while decreased activity leads to hypofibrinolysis, which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016] |
| UniProt Code: | P00750 |
| NCBI GenInfo Identifier: | 137119 |
| NCBI Gene ID: | 5327 |
| NCBI Accession: | P00750.1 |
| UniProt Secondary Accession: | P00750,Q15103, Q503B0, Q6PJA5, Q7Z7N2, Q86YK8, Q9BU99 Q9BZW1, A8K022, B2R8E8, |
| UniProt Related Accession: | P00750 |
| Molecular Weight: | 44,373 Da |
| NCBI Full Name: | Tissue-type plasminogen activator |
| NCBI Synonym Full Names: | plasminogen activator, tissue type |
| NCBI Official Symbol: | PLAT�� |
| NCBI Official Synonym Symbols: | TPA; T-PA�� |
| NCBI Protein Information: | tissue-type plasminogen activator |
| UniProt Protein Name: | Tissue-type plasminogen activator |
| UniProt Synonym Protein Names: | INN: Alteplase; INN: Reteplase |
| Protein Family: | Taurine--pyruvate aminotransferase |
| UniProt Gene Name: | PLAT�� |
