Human Agouti–Related Protein Recombinant Protein (CPRT1267)

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CPRT1267
€278

Description

Data SheetMSDS

AGRP Human Recombinant Protein

Name:AGRP Human
Synonyms:ART, AGRT, ASIP2, MGC118963, AGRP.
Introduction:Agouti–related protein is an endogenous antagonist of hypothalamic alpha–melanocortin receptors MC3R and MC4R with potent orexigenic activity. Although a complete deletion of the AGRP gene does not produce any significant metabolic phenotypes, reduction in AGRP expression by RNA interference is associated with increased metabolic rate alongwith reduced weight gain. In hypothalamus, it is produced by neurons in the medial portion of arcuate nucleus, which produce also the potent orexigenic peptide Neuropeptide Y (NP–Y). Another site of central AGRP production is the hypothalamic nucleus. AGRP encompasses 132 amino acid residues and its alpha–melanocortin inhibitingactivity results in a 34 amino acid cystine knot domain within the C–terminal (87–132) portion of the protein. Both AGRP and NP–Y expression was shown to be suppressed by leptin. Central administration of AGRP induces hyperphagia and increased gain in body weight in rodents, but may also exert metabolic effects even when hyperphagia is prevented. In the absence of hyperphagia, intracerebralventricular administration of AGRP caused significant increases in plasma leptin and insulin concentrations (twofold and 1.5–fold, respectively) and fat pad mass.In the periphery, AGRP mRNA was found in adrenal glands, lung, testis, ovary, skeletal muscle and adipose tissue in humans or rodents. In the adrenals, it was shown that AGRP antagonizes glucosteroid production mediated by MC4R. AGRP could then modulate locally the functions of some peripheral tissues such as adrenals.In human and rat serum, detectable levels of AGRP–like activity were reported in the lower picogram range. The serum AGRP levels were elevated in obese humans compared to lean controls and increased with fastingin rats.
Description:The Human Agouti–related protein is created as a recombinant protein with N–terminal fusion of His Tag.The Human Agouti–related protein His–Tagged Fusion Protein, produced in E. coli, is 14.4 kDa (calculated) protein containing 112 amino acid residues of the human AGRP and 16 additional amino acid residues – His Tag, thrombin cleavage site (highlighted).The AGRP is purified by proprietary chromatographic techniques.
Source:Escherichia Coli.
Pack size:10µg
Physical Appearance:Sterile Filtered White lyophilized (freeze–dried) powder.
Formulation:AGRP protein was lyophilized from 0.5mg/ml in 5mM TRIS, 25mM NaCl, pH 7.5.
Solubility:Add deionized water to prepare a workingstock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely.
Stability:Store lyophilized AGRP protein at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawingcycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Purity:Purity of Agouti–related protein recombinant human is >95% as determined by SDS–PAGE.
Amino Acid Sequence:MKHHHHHHHM LVPRGSAQMgLAPMEGIRRP DQALLPELPgLGLRAPLKKT TAEQAEEDLL QEAQALAEVL DLQDREPRSS RRCVRLHESC LGQQVPCCDP CATCYCRFFN AFCYCRKLGT AMNPCSRT.
Biological Activity:N/A
Usage:Assay Genie’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Uniprot:O00253

AGRP Human Recombinant Protein - Protein Information

UniProt Protein Function:AGRP: Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R. Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity. It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner. Genetic variations in AGRP may be a cause of obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
UniProt Protein Details:

Protein type:Secreted; Hormone; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16q22

Cellular Component: extracellular space; cell soma; Golgi lumen

Molecular Function:neuropeptide hormone activity; receptor binding

Biological Process: maternal process involved in pregnancy; hormone-mediated signaling; eating behavior; neuropeptide signaling pathway; photoperiodism; feeding behavior; adult feeding behavior; response to insulin stimulus

Disease: Obesity

NCBI Summary:This gene encodes an antagonist of the melanocortin-3 and melanocortin-4 receptor. It appears to regulate hypothalamic control of feeding behavior via melanocortin receptor and/or intracellular calcium regulation, and thus plays a role in weight homeostasis. Mutations in this gene have been associated with late on-set obesity. [provided by RefSeq, Dec 2009]
UniProt Code:O00253
NCBI GenInfo Identifier:4501995
NCBI Gene ID:181
NCBI Accession:NP_001129.1
UniProt Secondary Accession:O00253,O15459, Q2TBD9,
UniProt Related Accession:O00253
Molecular Weight:
NCBI Full Name:agouti-related protein
NCBI Synonym Full Names:agouti related neuropeptide
NCBI Official Symbol:AGRP  
NCBI Official Synonym Symbols:ART; AGRT; ASIP2  
NCBI Protein Information:agouti-related protein; agouti related protein homolog
UniProt Protein Name:Agouti-related protein
Protein Family:Agouti-related protein
UniProt Gene Name:AGRP  
UniProt Entry Name:AGRP_HUMAN
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Additional Information

Product type:
Recombinant Protein
Reactivity:
Human
Host Species:
E.Coli
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