Human CD22 Monoclonal Antibody (Alexa Fluor 488 Conjugated, Clone BU59)



Technical Manual


Product Name: Human CD22 Monoclonal Antibody (Alexa Fluor 488 Conjugated, Clone BU59)
Host Species: Mouse
Size: 50/100/200 Tests


CD22 is a 130 kD type I transmembrane glycoprotein also known as Siglec-2 and BL-CAM. It is a member of the immunoglobulin superfamily (sialoadhesion subgroup). CD22 is expressed in the cytoplasm of pro-B and pre-B cells, and on the surface of mature B and activated B cells, but not on plasma cells. CD22 is present in the B cell receptor complex and associates with SHP-1, Syk, Lck, Lyn, and phospholipase Cγ1. A primary function of CD22 is thought to be in limiting antigen receptor signaling by modulating B cell activation threshold. CD22 has been shown to bind to CD45RO and CD75, although the natural ligands for this molecule remain controversial.

Antibody Information:

Isotype: Mouse IgG1, κ
UniProt: P20273
Clone: BU59
Conjugation: AF488


Reactivity: Human
Tested Applications: FCM
Recommended Dilution: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µl staining volume or per 100 µl of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Storage Buffer: PBS with 0.05% Proclin300, 1% BSA
Storage: Keep as concentrated solution. Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents.
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