Human CLIC4 Recombinant Protein (RPPB3204)
- SKU:
- RPPB3204
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9Y696
Description
| Product Name: | Human CLIC4 Recombinant Protein |
| Product Code: | RPPB3204 |
| Size: | 20µg |
| Species: | Human |
| Target: | CLIC4 |
| Synonyms: | Chloride intracellular channel protein 4, Intracellular chloride ion channel protein p64H1, CLIC4, H1, huH1, p64H1, CLIC4L, MTCLIC, FLJ38640, DKFZp566G223. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The CLIC4 solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.1M NaCl, 1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MALSMPLNGL KEEDKEPLIE LFVKAGSDGE SIGNCPFSQR LFMILWLKGV VFSVTTVDLK RKPADLQNLA PGTHPPFITF NSEVKTDVNK IEEFLEEVLC PPKYLKLSPK HPESNTAGMD IFAKFSAYIK NSRPEANEAL ERGLLKTLQK LDEYLNSPLP DEIDENSMED IKFSTRKFLD GNEMTLADCN LLPKLHIVKV VAKKYRNFDI PKEMTGIWRY LTNAYSRDEF TNTCPSDKEV EIAYSDVAKR LTK |
Chloride intracellular channel 4 (CLIC4) belongs to the p64 family; CLIC4 is expressed in various tissues and exhibits an intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). CLIC4 is a 253 amino acid single-pass membrane protein which localizes to both the nucleus and the cytoplasm and contains one GST C-terminal domain. CLIC4 acts as a monomer which is able to form selective ion channels in target proteins, thus facilitating the transport of chloride and other ions. CLIC4 is believed to have a role in apoptosis and is able to translocate to the nucleus under stress conditions.
CLIC4 Human Recombinant fused with a�20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing�273 amino acids (1-253 a.a.) and having a molecular mass of 30.9kDa. The CLIC4 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CLIC4: Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell- surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical- basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis). Belongs to the chloride channel CLIC family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 1p36.11 Cellular Component: centrosome; cell surface; nuclear matrix; microvillus; cytoplasmic vesicle membrane; mitochondrion; intercellular junction; cytosol; actin cytoskeleton; microtubule cytoskeleton; apical part of cell; perinuclear region of cytoplasm; cytoplasm; plasma membrane; intracellular; midbody Molecular Function:chloride channel activity; protein binding; voltage-gated ion channel activity Biological Process: keratinocyte differentiation; vacuolar acidification; fertilization; branching morphogenesis of a tube; apoptosis; multicellular organism growth; establishment and/or maintenance of apical/basal cell polarity; chloride transport; angiogenesis; endothelial cell morphogenesis; cell differentiation; negative regulation of cell migration; regulation of cytoskeleton organization and biogenesis |
| NCBI Summary: | Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9Y696 |
| NCBI GenInfo Identifier: | 20141285 |
| NCBI Gene ID: | 25932 |
| NCBI Accession: | Q9Y696.4 |
| UniProt Secondary Accession: | Q9Y696,Q9UFW9, Q9UQJ6, |
| UniProt Related Accession: | Q9Y696 |
| Molecular Weight: | 253 |
| NCBI Full Name: | Chloride intracellular channel protein 4 |
| NCBI Synonym Full Names: | chloride intracellular channel 4 |
| NCBI Official Symbol: | CLIC4�� |
| NCBI Official Synonym Symbols: | H1; huH1; p64H1; CLIC4L; MTCLIC�� |
| NCBI Protein Information: | chloride intracellular channel protein 4; chloride intracellular channel 4 like; intracellular chloride ion channel protein p64H1 |
| UniProt Protein Name: | Chloride intracellular channel protein 4 |
| UniProt Synonym Protein Names: | Intracellular chloride ion channel protein p64H1 |
| Protein Family: | Chloride intracellular channel protein |
| UniProt Gene Name: | CLIC4�� |
| UniProt Entry Name: | CLIC4_HUMAN |
