Human FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart) ELISA Kit
The Human FABP3 (Fatty Acid Binding Protein 3) Muscle and Heart ELISA Kit is designed for the precise measurement of FABP3 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.FABP3 is a key protein involved in fatty acid metabolism and is predominantly found in muscle and heart tissues. Its levels can indicate muscle and heart damage, making it a valuable biomarker for studying conditions such as heart attacks, heart failure, and skeletal muscle diseases. This ELISA kit is an essential tool for researchers looking to investigate these conditions and develop potential treatments.
Product Name:
Human FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart) ELISA Kit
SKU:
HUES02467
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
