Human EIF2B1 Recombinant Protein (RPPB3423)
- SKU:
- RPPB3423
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q14232
Description
| Product Name: | Human EIF2B1 Recombinant Protein |
| Product Code: | RPPB3423 |
| Size: | 5µg |
| Species: | Human |
| Target: | EIF2B1 |
| Synonyms: | Translation initiation factor eIF-2B subunit alpha, eIF-2B GDP-GTP exchange factor subunit alpha, EIF2B1, EIF2BA, EIF2B. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The EIF2B1 solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.1M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MDDKELIEYF KSQMKEDPDM ASAVAAIRTL LEFLKRDKGE TIQGLRANLT SAIETLCGVD SSVAVSSGGE LFLRFISLAS LEYSDYSKCK KIMIERGELF LRRISLSRNK IADLCHTFIK DGATILTHAY SRVVLRVLEA AVAAKKRFSV YVTESQPDLS GKKMAKALCH LNVPVTVVLD AAVGYIMEKA DLVIVGAEGV VENGGIINKI GTNQMAVCAK AQNKPFYVVA ESFKFVRLFP LNQQDVPDKF KYKADTLKVA QTGQDLKEEH PWVDYTAPSL ITLLFTDLGV LTPSAVSDEL IKLYL |
EIF2B1 is one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), which is a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Phosphorylation of eIF2 inhibits GEF activity of EIF2B, an inhibition which requires the eIF2B1 subunit. Defects in eIF2B1 are a cause of leukoencephalopathy with vanishing white matter (VWM), a brain disease which is characterized by head trauma and motor deterioration.
EIF2B1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 325 amino acids (1-305 a.a.) and having a molecular mass of 35.8kDa.EIF2B1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | eIF2B-alpha: Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. Defects in EIF2B1 are a cause of leukodystrophy with vanishing white matter (VWM). VWM is a leukodystrophy that occurs mainly in children. Neurological signs include progressive cerebellar ataxia, spasticity, inconstant optic atrophy and relatively preserved mental abilities. The disease is chronic-progressive with, in most individuals, additional episodes of rapid deterioration following febrile infections or minor head trauma. While childhood onset is the most common form of the disorder, some severe forms are apparent at birth. A severe, early-onset form seen among the Cree and Chippewayan populations of Quebec and Manitoba is called Cree leukoencephalopathy. Milder forms may not become evident until adolescence or adulthood. Some females with milder forms of the disease who survive to adolescence exhibit ovarian dysfunction. This variant of the disorder is called ovarioleukodystrophy. Belongs to the eIF-2B alpha/beta/delta subunits family. |
| UniProt Protein Details: | Protein type:Translation; Translation initiation Chromosomal Location of Human Ortholog: 12q24.31 Cellular Component: cytoplasm; cytosol; eukaryotic translation initiation factor 2B complex; membrane; plasma membrane Molecular Function:guanyl-nucleotide exchange factor activity; protein binding; S-methyl-5-thioribose-1-phosphate isomerase activity; translation initiation factor activity Biological Process: cellular response to stimulus; methionine salvage; oligodendrocyte development; regulation of translational initiation; response to glucose stimulus; response to heat; response to peptide hormone stimulus; translational initiation Disease: Leukoencephalopathy With Vanishing White Matter |
| NCBI Summary: | This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. [provided by RefSeq, Oct 2009] |
| UniProt Code: | Q14232 |
| NCBI GenInfo Identifier: | 2494303 |
| NCBI Gene ID: | 1967 |
| NCBI Accession: | Q14232.1 |
| UniProt Secondary Accession: | Q14232,Q3SXP4, A6NLY9, B4DGX0, |
| UniProt Related Accession: | Q14232 |
| Molecular Weight: | 24,618 Da |
| NCBI Full Name: | Translation initiation factor eIF-2B subunit alpha |
| NCBI Synonym Full Names: | eukaryotic translation initiation factor 2B subunit alpha |
| NCBI Official Symbol: | EIF2B1�� |
| NCBI Official Synonym Symbols: | EIF2B; EIF2BA�� |
| NCBI Protein Information: | translation initiation factor eIF-2B subunit alpha |
| UniProt Protein Name: | Translation initiation factor eIF-2B subunit alpha |
| UniProt Synonym Protein Names: | eIF-2B GDP-GTP exchange factor subunit alpha |
| Protein Family: | Translation initiation factor |
| UniProt Gene Name: | EIF2B1�� |
| UniProt Entry Name: | EI2BA_HUMAN |
