Human Granzyme-B (CPRT1782)

SKU:
CPRT1782
€278

Description

Data SheetMSDS

GZMB Human Enzyme

Name:GZMB Human
Synonyms:Granzyme B, C11, CTLA-1, Cathepsin G-like 1, CTSGL1, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, Fragmentin-2, Granzyme-2, Human lymphocyte protein, HLP, SECT, T-cell serine protease 1-3E, CGL1, CSPB, CTLA1, GRB, GZMB, CCPI, CGL-1, CSP-B.
Introduction:Granzyme–B (GZMB) is essential for target cell lysis in cell–mediated immune responses. GZMB is related to an activation cascade of caspases responsible for apoptosis execution. GZMB cuts caspase–3, –7, –9 and 10 to activate enzymes mediatingapoptosis.
Description:GZMB Human Recombinant produced in E.Coli is a single, non–glycosylated polypeptide chain containing 248 amino acids (21–247 a.a.) and having a molecular mass of 27.8kDa.GZMB is fused to a 20 amino acid His–tag at N–terminus & purified by proprietary chromatographic techniques.
Source:E.coli.
Pack size:20µg
Physical Appearance:Sterile Filtered colorless solution.
Formulation:GZMB protein solution (1mg/ml) containing 20mM Tris–HCl buffer (pH 8.0) and 10% glycerol.
Solubility:N/A
Stability:Store at 4°C if entire vial will be used within 2–4 weeks. Store, frozen at –20°C for longer periods of time. For longterm storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze–thaw cycles.
Purity:Greater than 85% as determined by SDS–PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MIIGGHEAKP HSRPYMAYLM IWDQKSLKRC GGFLIQDDFV LTAAHCWGSS INVTLGAHNI KEQEPTQQFI PVKRPIPHPA YNPKNFSNDI MLLQLERKAK RTRAVQPLRL PSNKAQVKPgQTCSVAGWGQ TAPLGKHSHT LQEVKMTVQE DRKCESDLRH YYDSTIELCV GDPEIKKTSF KGDSGGPLVC NKVAQGIVSY GRNNGMPPRA CTKVSSFVHW IKKTMKRY.
Biological Activity:N/A
Usage:Assay Genie’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Uniprot:P10144

GZMB Human Enzyme - Protein Information

UniProt Protein Function:GZMB: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. By staphylococcal enterotoxin A (SEA) in peripheral blood leukocytes. Inactivated by the serine protease inhibitor diisopropylfluorophosphate. Belongs to the peptidase S1 family. Granzyme subfamily.
UniProt Protein Details:

Protein type:Apoptosis; EC 3.4.21.79; Protease

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: cytoplasm; cytosol; immunological synapse; intracellular membrane-bound organelle; membrane; nucleus

Molecular Function:protein binding; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: apoptosis; natural killer cell mediated cytotoxicity; protein processing

NCBI Summary:This gene encodes a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. [provided by RefSeq, Sep 2016]
UniProt Code:P10144
NCBI GenInfo Identifier:317373361
NCBI Gene ID:3002
NCBI Accession:P10144.2
UniProt Secondary Accession:P10144,Q8N1D2, Q9UCC1,
UniProt Related Accession:P10144
Molecular Weight:
NCBI Full Name:Granzyme B
NCBI Synonym Full Names:granzyme B
NCBI Official Symbol:GZMB
NCBI Official Synonym Symbols:C11; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
NCBI Protein Information:granzyme B
UniProt Protein Name:Granzyme B
UniProt Synonym Protein Names:C11; CTLA-1; Cathepsin G-like 1; CTSGL1; Cytotoxic T-lymphocyte proteinase 2; Lymphocyte protease; Fragmentin-2; Granzyme-2; Human lymphocyte protein; HLP; SECT; T-cell serine protease 1-3E
Protein Family:Granzyme
UniProt Gene Name:GZMB
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Additional Information

Product type:
Enzyme
Reactivity:
Human
Host Species:
E.Coli
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