Human GLRX3 Recombinant Protein (RPPB1703)
- SKU:
- RPPB1703
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- O76003
- Research Area:
- Enzymes
Description
| Product Name: | Human GLRX3 Recombinant Protein |
| Product Code: | RPPB1703 |
| Size: | 25µg |
| Species: | Human |
| Target: | GLRX3 |
| Synonyms: | Glutaredoxin-3, Thioredoxin-like protein 2, PKC-interacting cousin of thioredoxin, PKC-theta-interacting protein, PKCq-interacting protein, GLRX3, PICOT, TXNL2, GRX3, GRX4, GLRX4, TXNL3, FLJ11864, bA500G10.4. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | GLRX3 solution containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol and1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAAGAAEAAV AAVEEVGSAG QFEELLRLKA KSLLVVHFWA PWAPQCAQMN EVMAELAKEL PQVSFVKLEA EGVPEVSEKY EISSVPTFLF FKNSQKIDRL DGAHAPELTK KVQRHASSGS FLPSANEHLK EDLNLRLKKL THAAPCMLFM KGTPQEPRCG FSKQMVEILH KHNIQFSSFD IFSDEEVRQG LKAYSSWPTY PQLYVSGELI GGLDIIKELE ASEELDTICP KAPKLEERLK VLTNKASVML FMKGNKQEAK CGFSKQILEI LNSTGVEYET FDILEDEEVR QGLKAYSNWP TYPQLYVKGE LVGGLDIVKE LKENGELLPI LRGEN |
Glutaredoxin belongs to the thiol-disulfide oxidoreductase family. Glutraredoxins catalyze the reversible reduction of protein-glutathionyl mixed disulfides to free sulfhydryl groups though a monothiol mechanism. Glutaredoxin-3 (GRX3) is an important protein involved in the regulation of signal transduction, for example during immune cell activation and development of cardiac hypertrophy, apparently in response to redox signals. GRX3 interacts with PRKCQ. GRX3 may have a role in the regulation the function of the thioredoxin system. GRX3 is expressed in the heart, spleen, testis and, to a lower extent, in the thymus and peripheral blood leukocytes. GRX3 is weakly expressed in lung, placenta, colon and small intestine.
GLRX3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 355 amino acids (1-335 a.a.) and having a molecular mass of 39.6kDa. GLRX3 is fused to a 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GLRX3: Critical negative regulator of cardiac hypertrophy and a positive inotropic regulator. May play a role in regulating the function of the thioredoxin system. Does not posses any thyoredoxin activity since it lacks the conserved motif that is essential for catalytic activity. |
| UniProt Protein Details: | Protein type:Oxidoreductase Chromosomal Location of Human Ortholog: 10q26 Cellular Component: cell cortex; Z disc Molecular Function:protein binding; electron carrier activity; protein kinase C binding; metal ion binding; iron-sulfur cluster binding; protein disulfide oxidoreductase activity Biological Process: cell redox homeostasis; regulation of the force of heart contraction |
| NCBI Summary: | This gene encodes a member of the glutaredoxin family. Glutaredoxins are oxidoreductase enzymes that reduce a variety of substrates using glutathione as a cofactor. The encoded protein binds to and modulates the function of protein kinase C theta. The encoded protein may also inhibit apoptosis and play a role in cellular growth, and the expression of this gene may be a marker for cancer. Pseudogenes of this gene are located on the short arm of chromosomes 6 and 9. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2010] |
| UniProt Code: | O76003 |
| NCBI GenInfo Identifier: | 37087933 |
| NCBI Gene ID: | 10539 |
| NCBI Accession: | O76003.2 |
| UniProt Related Accession: | O76003 |
| Molecular Weight: | Observed: 37 kDaPredicted: 38 kDa |
| NCBI Full Name: | Glutaredoxin-3 |
| NCBI Synonym Full Names: | glutaredoxin 3 |
| NCBI Official Symbol: | GLRX3�� |
| NCBI Official Synonym Symbols: | GRX3; GRX4; GLRX4; PICOT; TXNL2; TXNL3�� |
| NCBI Protein Information: | glutaredoxin-3 |
| UniProt Protein Name: | Glutaredoxin-3 |
| UniProt Synonym Protein Names: | PKC-interacting cousin of thioredoxin; PICOT; PKC-theta-interacting protein; PKCq-interacting protein; Thioredoxin-like protein 2 |
| Protein Family: | Glutaredoxin |
| UniProt Gene Name: | GLRX3�� |
| UniProt Entry Name: | GLRX3_HUMAN |
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