Human GroEL (HSP60) (27-573 a.a.) Recombinant Protein (CPRT5221)

SKU:
CPRT5221
€278

Description

Data SheetMSDS

GROEL (27-573) Human Recombinant Protein

Name:GROEL (27-573) Human
Synonyms:CPN60, GROEL, HLD4, HSP-60, HSP60, HSP65, HuCHA60, SPG13, Chaperonin 60, 60 kDa chaperonin, P60 lymphocyte protein, 60 kDa heat shock protein, mitochondrial.
Introduction:GroEL, HSP60 is a chaperonin located in the mitochondria which is responsible for the transportation & refoldingof proteins from the cytoplasm directly into the mitochondrial matrix. GroEL is regulated by the HSP10 cochaperonin, which is a single heptameric protein ringhaving a molecular mass of 10 kDa which form a unique complex with HSP60. HSP10, GroES coordinates the ATPase activity of the HSP60 subunits in order to allow the release of bound polypeptide in a manner that is productive for its correct folding.
Description:GROEL Human Recombinant produced in E.Coli is a single, non–glycosylated polypeptide chain containing 572 amino acids (27–573 a.a.) and having a molecular mass of 60kDa.GROEL is fused to a 25 amino acid His–tag at N–terminus & purified by proprietary chromatographic techniques.
Source:Escherichia Coli.
Pack size:10µg
Physical Appearance:Sterile filtered colorless solution.
Formulation:GROEL protein solution (0.5mg/ml) contains 20mM Tris–HCl buffer, (pH 8.0) 0.1M NaCl and 10% glycerol.
Solubility:N/A
Stability:Store at 4°C if entire vial will be used within 2–4 weeks.Store, frozen at –20°C for longer periods of time. For longterm storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze–thaw cycles.
Purity:Greater than 90.0% as determined by SDS–PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMAKDVK FGADARALML QGVDLLADAV AVTMGPKGRT VIIEQSWGSP KVTKDGVTVA KSIDLKDKYK NIGAKLVQDV ANNTNEEAGD GTTTATVLAR SIAKEGFEKI SKGANPVEIR RGVMLAVDAV IAELKKQSKP VTTPEEIAQV ATISANGDKE IGNIISDAMK KVGRKGVITV KDGKTLNDEL EIIEGMKFDR GYISPYFINT SKGQKCEFQD AYVLLSEKKI SSIQSIVPAL EIANAHRKPL VIIAEDVDGE ALSTLVLNRL KVGLQVVAVK APGFGDNRKN QLKDMAIATgGAVFGEEGLT LNLEDVQPHD LGKVGEVIVT KDDAMLLKGK GDKAQIEKRI QEIIEQLDVT TSEYEKEKLN ERLAKLSDGV AVLKVGGTSD VEVNEKKDRV TDALNATRAA VEEGIVLGGgCALLRCIPAL DSLTPANEDQ KIGIEIIKRT LKIPAMTIAK NAGVEGSLIV EKIMQSSSEV GYDAMAGDFV NMVEKGIIDP TKVVRTALLD AAGVASLLTT AEVVVTEIPK EEKDPGMGAM GGMGGGMGGgMF.
Biological Activity:N/A
Usage:Assay Genie’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Uniprot:P10809

GROEL (27-573) Human Recombinant Protein - Protein Information

UniProt Protein Function:HSP60: Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. Interacts with HRAS. Interacts with HBV protein X and HTLV-1 protein p40tax. Belongs to the chaperonin (HSP60) family.
UniProt Protein Details:

Protein type:Chaperone; Mitochondrial

Chromosomal Location of Human Ortholog: 2q33.1

Cellular Component: extracellular space; cell surface; protein complex; mitochondrion; early endosome; coated pit; cytosol; secretory granule; membrane; mitochondrial matrix; cytoplasm; mitochondrial inner membrane; plasma membrane; lipopolysaccharide receptor complex; cyclin-dependent protein kinase activating kinase holoenzyme complex

Molecular Function:protein binding; p53 binding; lipopolysaccharide binding; ubiquitin protein ligase binding; chaperone binding; ATPase activity; double-stranded RNA binding; unfolded protein binding; DNA replication origin binding; single-stranded DNA binding; ATP binding

Biological Process: B cell proliferation; caspase activation; B cell activation; T cell activation; protein stabilization; viral reproduction; positive regulation of apoptosis; positive regulation of interleukin-12 production; protein maturation; isotype switching to IgG isotypes; positive regulation of interleukin-6 production; B cell cytokine production; positive regulation of interleukin-10 production; response to unfolded protein; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interferon-gamma production; ’de novo’ protein folding; protein refolding; positive regulation of T cell activation; positive regulation of T cell mediated immune response to tumor cell; chaperone-mediated protein complex assembly; positive regulation of interferon-alpha production; negative regulation of apoptosis; positive regulation of macrophage activation

Disease: Spastic Paraplegia 13, Autosomal Dominant; Leukodystrophy, Hypomyelinating, 4

NCBI Summary:This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]
UniProt Code:P10809
NCBI GenInfo Identifier:129379
NCBI Gene ID:3329
NCBI Accession:P10809.2
UniProt Secondary Accession:P10809,Q38L19, Q9UCR6, B2R5M6, B7Z712,
UniProt Related Accession:P10809
Molecular Weight:17,100 Da
NCBI Full Name:60 kDa heat shock protein, mitochondrial
NCBI Synonym Full Names:heat shock 60kDa protein 1 (chaperonin)
NCBI Official Symbol:HSPD1
NCBI Official Synonym Symbols:HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
NCBI Protein Information:60 kDa heat shock protein, mitochondrial; chaperonin 60; 60 kDa chaperonin; heat shock protein 65; P60 lymphocyte protein; mitochondrial matrix protein P1; short heat shock protein 60 Hsp60s1
UniProt Protein Name:60 kDa heat shock protein, mitochondrial
UniProt Synonym Protein Names:60 kDa chaperonin; Chaperonin 60; CPN60; Heat shock protein 60; HSP-60; Hsp60; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein
Protein Family:60 kDa heat shock protein
UniProt Gene Name:HSPD1
UniProt Entry Name:CH60_HUMAN
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Additional Information

Product type:
Recombinant Protein
Reactivity:
Human
Host Species:
E.Coli
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