Human Inosine-5’-Monophosphate Dehydrogenase (IMPDH) Inhibitor Screening Assay Kit (BN00728)

(No reviews yet) Write a Review
SKU:
BN00728
  • Human Inosine-5'-Monophosphate Dehydrogenase (IMPDH) Inhibitor Screening Assay Kit (BN00728)
  • Human Inosine-5'-Monophosphate Dehydrogenase (IMPDH) Inhibitor Screening Assay Kit (BN00728)
$825.63

Description

ELISA Kit Technical ManualMSDS

Human Inosine-5'-Monophosphate Dehydrogenase (IMPDH) Inhibitor Screening Assay Kit

Inosine monophosphate dehydrogenase, IMPDH, (E.C. 1.1.1.205) is the rate-limiting enzyme in de novo guanine nucleotide biosynthesis and it is essential for lymphocyte proliferation. IMPDH oxidizes inosine 5'-monophosphate (IMP) to xanthine 5'-monophosphate (XMP) using NAD as a cofactor. It plays a critical role in cell growth and in the malignancy of some tumors such as pancreatic, colon and bladder cancers. IMPDH2 is the predominant isoform of IMPDH and is specifically linked to a wide range of cancers and lymphocyte proliferation. IMPDH is recognized as a validated target as antiviral, antiparasitic, antimicrobial, antileukemic and immunosuppressive agents. In BioVison's IMPDH inhibitor screening assay, IMP is oxidized by IMPDH producing a series of intermediates, which react with the probe to generate a colorimetric signal (OD 450 nm). The signal is directly proportional to the IMPDH activity and has a very low background. In the presence of a IMPDH inhibitor such as mycophenolic acid (MPA), the reaction is arrested, thus decreasing the signal. Assay Genie's IMPDH assay is fast, sensitive and reproducible and is suitable for functional assays, high-throughput screening and preclinical studies in drug discovery.

Figure A shows the reaction kinetics in the presence and absence of the IMPDH2 enzyme. B. Inhibition of IMPDH2 enzymatic activity using MPA (IC 50 : 59 nM).

Key Information Description

Product SKU

BN00728

Size

100 assays

Kit Summary

  • Detection method- Colorimetric (OD 450 nm)
  • Application-Screening/characterizing/studying IMPDH inhibitors/activators

Detection Method

Colorimetric (OD 450 nm)

Species Reactivity

Human

Applications

Screening/characterizing/studying IMPDH inhibitors/activators

Features and Benefits

  • Simple and reliable test to screen IMPDH inhibitors/activators
  • High-throughput compatible Includes Inhibitor Control (MPA)

Kit Components

  • Assay Buffer
  • IMPDH Substrate
  • IMPDH Detection
  • IMPDH2 Enzyme
  • MPA (10 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

View AllClose