Human Nanog, His Tag Recombinant Protein (CPRT4053)

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Data SheetMSDS

NANOgHuman, His Recombinant Protein

Name:NANOgHuman, His
Synonyms:NANOG, Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog.
Introduction:NANOgis a multidomain homeobox transcription factor which functions to maintain the undifferentiated state of pluripotent stem cells. NANOgexpression counteracts the differentiation–promotingsignals induced by the extrinsic factors LIF, Stat3 and BMP. Once NANOgexpression is downregulated, cell differentiation can proceed. Proteins which regulate NANOgexpression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.
Description:NANOgHuman Recombinant produced in E.coli is a single, non–glycosylated polypeptide chain containing 174 amino acids (1–154) and having a molecular mass of 19.6 kDa.The NANOgis fused to a 20 amino acid His–tag at N–terminus and purified by proprietary chromatographic techniques.
Source:Escherichia Coli.
Pack size:20µg
Physical Appearance:Sterile Filtered colorless solution.
Formulation:NANOgprotein (0.25mg/ml) is supplied in 20mM Tris–HCL, pH–8, 1mM DTT and 20% Glycerol.
Stability:Store at 4°C if entire vial will be used within 2–4 weeks. Store, frozen at –20°C for longer periods of time. For longterm storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze–thaw cycles.
Purity:Greater than 90.0% as determined by SDS–PAGE.
Biological Activity:N/A
Usage:Assay Genie’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

NANOgHuman, His Recombinant Protein - Protein Information

UniProt Protein Function:NANOG: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5’-TAAT[GT][GT]-3’ or 5’-[CG][GA][CG]C[GC]ATTAN[GC]-3’. When overexpressed, promotes cells to enter into S phase and proliferation. Interacts with SMAD1 and SALL4. Expressed in testicular carcinoma and derived germ cell tumors. Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. Belongs to the Nanog homeobox family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription factor; DNA-binding; Cell development/differentiation

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function:DNA binding; chromatin binding; transcription factor activity; transcription corepressor activity

Biological Process: response to retinoic acid; gonad development; transcription, DNA-dependent; somatic stem cell maintenance; stem cell division; endodermal cell fate specification; stem cell maintenance; negative regulation of transcription from RNA polymerase II promoter; positive regulation of mitotic cell cycle; embryonic pattern specification; mesodermal cell fate commitment; negative regulation of cell fate commitment; negative regulation of BMP signaling pathway; cell proliferation; regulation of transcription, DNA-dependent; regulation of gene expression; regulation of cell differentiation; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; cell differentiation

NCBI Summary:The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
UniProt Code:Q9H9S0
NCBI GenInfo Identifier:118573073
NCBI Gene ID:79923
NCBI Accession:Q9H9S0.2
UniProt Secondary Accession:Q9H9S0,Q2TTG0, Q6JZS5, D3DUU4,
UniProt Related Accession:Q9H9S0
Molecular Weight:305
NCBI Full Name:Homeobox protein NANOG
NCBI Synonym Full Names:Nanog homeobox
NCBI Official Symbol:NANOG  
NCBI Protein Information:homeobox protein NANOG; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48
UniProt Protein Name:Homeobox protein NANOG
UniProt Synonym Protein Names:Homeobox transcription factor Nanog; hNanog
Protein Family:NANOG neighbor homeobox
UniProt Gene Name:NANOG  
UniProt Entry Name:NANOG_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host Species:
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