Human NKp46/NCR1/CD335 PharmaGenie ELISA Kit (SBRS0927)

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Human NKp46/NCR1/CD335 PharmaGenie ELISA - Kit Data

Product SKU:SBRS0927
Application:This ELISA kit shows no cross-reactivity with the following cytokines tested: human Adenosine Deaminase/ADA, AIF, AKR1C4 , ASAH2, BCL-2, BID, Calreticulin, Calreticulin-2, CD314 (NKG2D), CD39L3/ENTPD3, CD51, CD99-L2, CDC25B, Cerberus 1, CHST2, Cochlin, CRELD2, DC-SIGNR/CD299, eNOS, ENPP2/Autotaxin, FABP4, Fc epsilon RI, FGF R5, GALNT2, GALNT3, GIF, GPR111, beta-Glucuronidase/GUSB, Inhibin A, LILRB4/CD85k/ILT3, Neuroglycan C/CSPG5, Neuronal Pentraxin R/NPTXR, ROR2, SCCA2, Siglec-2/CD22, SIRP alpha/CD172a, SorCS1, Trypsin 1/PRSS1, Trypsin 3/PRSS3.
Gene ID:9437
Gene Names:LY94 / NCR1
Synonyms:Natural cytotoxicity triggering receptor 1 (Lymphocyte antigen 94 homolog) (NK cell-activating receptor) (Natural killer cell p46-related protein) (NK-p46) (NKp46) (hNKp46) (CD antigen CD335)
Target Species:Human
Compatible Sample Types:Cell Culture Supernatants, Plasma, Serum
Design Principle:Sandwich-based
Method of Detection:Colorimetric
Range:4.5 pg/ml - 1000 pg/ml
Sensitivity:4.5 pg/ml
Recommended Dilution:5 fold
Storage/Stability:The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Kit Components:

1.Pre-Coated 96-well Strip Microplate
2.Wash Buffer
3.Stop Solution
4.Assay Diluent(s)
5.Lyophilized Standard
6.Biotinylated Detection Antibody
7.Streptavidin-Conjugated HRP
8.TMB One-Step Substrate

Other materials and equipment required:

The Assay Genie Human NKp46/NCR1/CD335 ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ul volumes
  • Adjustable 1-25 ul pipettes for reagent preparation
  • 100 ul and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Kit Protocol:

1.Prepare all reagents, samples and standards as instructed in the manual.
2.Add 100 ul of standard or sample to each well.
3.Incubate 2.5 h at RT or O/N at 4°C.
4.Add 100 ul of prepared biotin antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 ul of prepared Streptavidin solution to each well.
7.Incubate 45 min at RT.
8.Add 100 ul of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 ul of Stop Solution to each well.
11.Read at 450 nm immediately.