Human NDFIP1 Recombinant Protein (RPPB4074)
- SKU:
- RPPB4074
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9BT67
Frequently bought together:
Description
| Product Name: | Human NDFIP1 Recombinant Protein |
| Product Code: | RPPB4074 |
| Size: | 20µg |
| Species: | Human |
| Target: | NDFIP1 |
| Synonyms: | N4WBP5, NEDD4 family-interacting protein 1, Breast cancer-associated protein SGA-1M, NEDD4 WW domain-binding protein 5, Putative MAPK-activating protein PM13, Putative NF-kappa-B-activating protein 164, Putative NFKB and MAPK-activating protein,NDFIP1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The NDFIP1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.1M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMALALAA LAAVEPACGS RYQQLQNEEE SGEPEQAAGD APPPYSSISA ESAAYFDYKD ESGFPKPPSY NVATTLPSYD EAERTKAEAT IPLVPGRDED FVGRDDFDDA DQLRIGNDG |
Nedd4 Family Interacting Protein 1 (NDFIP1) is a part of a small group of evolutionarily conserved proteins having 3 ransmembrane domains. NDFIP1 is a possible target for ubiquitination by the Nedd4 family of proteins. NDFIP, which is a member of a family of integral Golgi membrane proteins, also modulates EGFR signaling through multiple pathways.
NDFIP1 Human Recombinant produced in E. coli is. a single polypeptide chain containing 139 amino acids (1-116) and having a molecular mass of 14.8kDa. NDFIP1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | NDFIP1: Activates HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cells-mediated inflammation by activating ITCH and thus controlling JUNB degradation. In cortical neurons, mediates the ubiquitination of SLC11A2/DMT1 by NEDD4L, leading to down-regulation of the divalent metal transporter and protection of the cells from cobalt and iron toxicity. Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1-to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Adaptor/scaffold; Membrane protein, multi-pass; Membrane protein, integralChromosomal Location of Human Ortholog: 5q31.3Cellular Component: Golgi membrane; perinuclear region of cytoplasm; extracellular region; integral to membrane; endosome membrane; cell cortexMolecular Function: protein binding; signal transducer activity; WW domain bindingBiological Process: negative regulation of interleukin-4 production; positive regulation of I-kappaB kinase/NF-kappaB cascade; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; cellular iron ion homeostasis; regulation of lymphocyte differentiation; regulation of myeloid leukocyte differentiation; signal transduction; negative regulation of isotype switching to IgE isotypes; negative regulation of T cell proliferation; negative regulation of T-helper 2 type immune response; positive regulation of protein ubiquitination; negative regulation of inflammatory response; positive regulation of protein catabolic process; negative regulation of protein transport; negative regulation of transporter activity; regulation of isotype switching to IgG isotypes |
| UniProt Protein Details: | |
| NCBI Summary: | The protein encoded by this gene belongs to a small group of evolutionarily conserved proteins with three transmembrane domains. It is a potential target for ubiquitination by the Nedd4 family of proteins. This protein is thought to be part of a family of integral Golgi membrane proteins. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9BT67 |
| NCBI GenInfo Identifier: | 74733098 |
| NCBI Gene ID: | 80762 |
| NCBI Accession: | Q9BT67.1 |
| UniProt Secondary Accession: | Q9BT67,Q658T8, Q8N2E3, Q8N2F9, B2RDB8, D3DQF0 |
| UniProt Related Accession: | Q9BT67 |
| Molecular Weight: | 24,899 Da |
| NCBI Full Name: | NEDD4 family-interacting protein 1 |
| NCBI Synonym Full Names: | Nedd4 family interacting protein 1 |
| NCBI Official Symbol: | NDFIP1�� |
| NCBI Official Synonym Symbols: | N4WBP5�� |
| NCBI Protein Information: | NEDD4 family-interacting protein 1; Nedd4 WW domain-binding protein 5; putative MAPK-activating protein PM13; breast cancer-associated protein SGA-1M; putative NFKB and MAPK-activating protein; putative NF-kappa-B-activating protein 164 |
| UniProt Protein Name: | NEDD4 family-interacting protein 1 |
| UniProt Synonym Protein Names: | Breast cancer-associated protein SGA-1M; NEDD4 WW domain-binding protein 5; Putative MAPK-activating protein PM13; Putative NF-kappa-B-activating protein 164; Putative NFKB and MAPK-activating protein |
| Protein Family: | NEDD4 family-interacting protein |
| UniProt Gene Name: | NDFIP1�� |
| UniProt Entry Name: | NFIP1_HUMAN |
