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Human Phospho-ATR (T1989) and Total ATR PharmaGenie ELISA Kit (SBRS1756)

SKU:
SBRS1756
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
  • Human Phospho-ATR T1989 and Total ATR PharmaGenie ELISA Kit SBRS1756
  • Human Phospho-ATR T1989 and Total ATR PharmaGenie ELISA Kit SBRS1756
€820
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Description

Human Phospho-ATR (T1989) and Total ATR PharmaGenie ELISA Kit (SBRS1756)

Product SKU:SBRS1756
Size:96T
Application:This ELISA kit recognizes Human ATR phosphorylated at site Threonnine-1989 as well as total ATR.
Uniprot:Q13535
Gene ID:545
Gene Names:ATR / FRP1
Synonyms:Serine/threonine-protein kinase ATR (EC 2.7.11.1) (Ataxia telangiectasia and Rad3-related protein) (FRAP-related protein 1)
Storage/Stability:Upon receipt, the kit should be stored at -20°C. Please use within 6 months from the date of shipment.
  1. Pre-Coated 96-well Strip Microplate
  2. Wash Buffer
  3. Anti-Phospho Antibody
  4. HRP-Conjugated Secondary Antibody
  5. Assay Diluent
  6. TMB One-Step Substrate
  7. Stop Solution
  8. Lysis Buffer
  9. Positive Control Sample

Other materials and equipment required:

The Assay Genie Human Phospho-ATR (T1989) and Total ATR PharmaGenie ELISA Kit (SBRS1756) will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 ul of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 ul of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 ul of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ul of Stop Solution to each well.
  11. Read at 450 nm immediately.
Product ImageT47D cells were untreated or treated with UV. Cell lysates were analyzed using this phosphoELISA and Western Blot.
Product ImageT47D cells were exposed to 50J/m2 of UV light followed by a 4 hours recovery period. Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA

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