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Human Phospho-DDR1 (Y792) and Total DDR1 PharmaGenie ELISA Kit (SBRS1784)

SKU:
SBRS1784
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
  • Human Phospho-DDR1 Y792 and Total DDR1 PharmaGenie ELISA Kit SBRS1784
  • Human Phospho-DDR1 Y792 and Total DDR1 PharmaGenie ELISA Kit SBRS1784
€820
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Description

Human Phospho-DDR1 (Y792) and Total DDR1 PharmaGenie ELISA Kit (SBRS1784)

Product SKU:SBRS1784
Size:96T
Application:This ELISA kit recognizes Human DDR1 phosphorylated at site Tyrosine-792 as well as total DDR1.
Uniprot:Q08345
Gene ID:780
Gene Names:CAK / DDR1 / EDDR1 / NEP / NTRK4 / PTK3A / RTK6 / TRKE
Synonyms:Epithelial discoidin domain-containing receptor 1 (Epithelial discoidin domain receptor 1) (EC 2.7.10.1) (CD167 antigen-like family member A) (Cell adhesion kinase) (Discoidin receptor tyrosine kinase) (HGK2) (Mammary carcinoma kinase 10) (MCK-10) (Protein-tyrosine kinase 3A) (Protein-tyrosine kinase RTK-6) (TRK E) (Tyrosine kinase DDR) (Tyrosine-protein kinase CAK) (CD antigen CD167a)
Storage/Stability:Upon receipt, the kit should be stored at -20°C. Please use within 6 months from the date of shipment.
  1. Pre-Coated 96-well Strip Microplate
  2. Wash Buffer
  3. Anti-Phospho Antibody
  4. HRP-Conjugated Secondary Antibody
  5. Assay Diluent
  6. TMB One-Step Substrate
  7. Stop Solution
  8. Lysis Buffer
  9. Positive Control Sample

Other materials and equipment required:

The Assay Genie Human Phospho-DDR1 (Y792) and Total DDR1 PharmaGenie ELISA Kit (SBRS1784) will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 ul of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 ul of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 ul of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ul of Stop Solution to each well.
  11. Read at 450 nm immediately.
Product ImageJurkat cells were treated with Calyculin A and Pervanadate. Cell lysates were analyzed using this phosphoELISA and Western Blot.
Product ImageJurkat cells were treated with Pervanadate. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.

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