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Human Phospho-I-kappa B alpha (S32) and Total I-kappa B alpha PharmaGenie ELISA Kit (SBRS1858)

SKU:
SBRS1858
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
Sandwich
  • Human Phospho-I-kappa B alpha S32 and Total I-kappa B alpha PharmaGenie ELISA Kit SBRS1858
  • Human Phospho-I-kappa B alpha S32 and Total I-kappa B alpha PharmaGenie ELISA Kit SBRS1858
€820
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Description

Human Phospho-I-kappa B alpha (S32) and Total I-kappa B alpha PharmaGenie ELISA Kit (SBRS1858)

Assay Genie Phospho-IƒŽºB-alpha (S32) and Total IƒŽºB-alpha ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated IƒŽºB-alpha protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis. This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-IƒŽºB-alpha and total IƒŽºB-alpha. An anti-pan IƒŽºB-alpha antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and IƒŽºB-alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-IƒŽºB-alpha (S32) antibody is used to detect phosphorylated IƒŽºB-alpha or biotinylated anti-IƒŽºB-alpha antibody is used to detect pan IƒŽºB-alpha. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IƒŽºB-alpha (S32) or pan IƒŽºB-alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.