Human Phospho-LAT (Y161) and Total LAT PharmaGenie ELISA Kit (SBRS1877)

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Human Phospho-LAT (Y161) and Total LAT PharmaGenie ELISA - Kit Information

Assay Genie Phospho-LAT (Tyr161) and Total LAT ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated LAT protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis. This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-LAT and total LAT. An anti-pan LAT antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and LAT present in a sample is bound to the wells by the immobilized antibody. The wells are washed and mouse anti-LAT (Tyr161) antibody is used to detect phosphorylated LAT and pan LAT. After washing away unbound antibody, HRP-conjugated anti-mouse IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LAT (Tyr161) or pan LAT bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Human Phospho-LAT (Y161) and Total LAT PharmaGenie ELISA - Kit Data

Product SKU:SBRS1877
Application:The antibody pair provided in this kit recognizes human LAT phosphorylated at site Tyrosine-161 and total LAT.
Gene ID:8140
Gene Names:CD98LC / LAT1 / MPE16 / SLC7A5
Pathway:T Cell Receptor
Synonyms:Large neutral amino acids transporter small subunit 1 (4F2 light chain) (4F2 LC) (4F2LC) (CD98 light chain) (Integral membrane protein E16) (L-type amino acid transporter 1) (hLAT1) (Solute carrier family 7 member 5) (y+ system cationic amino acid transporter)
Target Species:Human
Compatible Sample Types:Cell Lysates, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Colorimetric
Storage/Stability:Upon receipt, the kit should be stored at -20°C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4°C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge, and store at -20°C. Item D, store at 2-8°C for up to one month (store at -20°C for up to 6 months, avoid repeated freeze-thaw cycles). Reconstituted Positive Control (Item K) should be stored at -70°C.

Human Phospho-LAT (Y161) and Total LAT PharmaGenie ELISA Kit (SBES1877) - Product Images

Product ImageJurkat cells were treated with Pervanadate. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.

Kit Components:

1.Pre-Coated 96-well Strip Microplate
2.Wash Buffer
3.Anti-Phospho Antibody
4.Anti-Pan Antibody
5.HRP-Conjugated Secondary Antibody
6.Streptavidin-Conjugated HRP
7.Assay Diluent
8.TMB One-Step Substrate
9.Stop Solution
10.Lysis Buffer
11.Positive Control Sample

Other materials and equipment required:

The Assay Genie Human Phospho-LAT (Y161) and Total LAT ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm

Kit Protocol:

1.Prepare all reagents and samples as instructed in the manual.
2.Add 100 ul of sample or positive control to each well.
3.Incubate 2.5 h at RT or O/N at 4 °C.
4.Add 100 ul of prepared primary antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 ul of prepared 1X HRP-Streptavidin to each well.
7.Incubate 1 h at RT.
8.Add 100 ul of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 ul of Stop Solution to each well.
11.Read at 450 nm immediately.