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Human RBP-4 PharmaGenie ELISA Kit (SBRS0118)

SKU:
SBRS0118
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
Competitive
€649
Frequently bought together:

Description

Human RBP-4 PharmaGenie ELISA - Kit Information

RBP4 (retinol binding protein-4) is an adipocyte-secreted molecule that is elevated in the serum before the development of frank diabetes. In obesity and type 2 diabetes, expression of the GLUT4 glucose transporter is decreased selectively in adipocytes. Adipose-specific Glut4 knockout mice show insulin resistance secondarily in muscle and liver. Recent studies have shown that serum RBP4 levels are elevated in insulin-resistant mice and humans with obesity and type 2 diabetes. RBP4 levels are normalized by rosiglitazone, an insulin-sensitizing drug. Transgenic overexpression of human RBP4 or injection of recombinant RBP4 in normal mice causes insulin resistance. Conversely, genetic deletion of RBP4 enhances insulin sensitivity. Fenretinide, a synthetic retinoid that increases urinary excretion of RBP4, normalizes serum RBP4 levels and improves insulin resistance and glucose intolerance in mice with obesity induced by a high-fat diet. Increasing serum RBP4 induces hepatic expression of the gluconeogenic enzyme phosphoenolpyruvate carboxykinase (PEPCK) and impairs insulin signalling in muscle. Thus, RBP4 is an adipocyte-derived 'signal' that may contribute to the pathogenesis of type 2 diabetes. Lowering RBP4 could be a new strategy for treating type 2 diabetes. Thus, RBP4 appears to identify insulin resistance and associated cardiovascular risk factors in subjects with varied clinical presentations.

Human RBP-4 PharmaGenie ELISA - Kit Data

Product Sample Types Method
Human RBP-4 PharmaGenie ELISA Kit (SBRS0118)Cell Culture Supernatants and SerumCompetitive
Human RBP-4 PharmaGenie ELISA Kit (SBRS1032)Cell Culture Supernatants, Plasma and SerumSandwich
Product SKU:SBRS0118
Size:96T
Application:This kit detects RBP4 (183aa) and all other active forms including RBP4-182,181,179,176. Cross Reactivity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II, NPY and APC.
Uniprot:P02753
Gene ID:5950
Gene Names:PRO2222 / RBP4
Synonyms:Retinol-binding protein 4 (Plasma retinol-binding protein) (PRBP) (RBP) [Cleaved into: Plasma retinol-binding protein(1-182) Plasma retinol-binding protein(1-181) Plasma retinol-binding protein(1-179) Plasma retinol-binding protein(1-176)]
Target Species:Human, Mouse, Rat
Compatible Sample Types:Cell Culture Supernatants and Serum
Design Principle:Competition-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Quantitative
Range:0.1-1,000 ng/ml
Sensitivity:460 pg/ml
Recommended Dilution:Human: 4X, Mouse: 2X
Storage/Stability:Standard, Biotinylated Retinol Binding Protein 4 peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Kit Components:

1.Pre-Coated 96-well Strip Microplate
2.Wash Buffer
3.Standard Peptide
4.Assay Diluent(s)
5.Biotinylated Peptide
6.HRP-Streptavidin
7.TMB One-Step Substrate
8.Stop Solution
9.Assay Diagram
10.Positive Control Sample
11.Capture Antibody
12.Technical Manual

Other materials and equipment required:

The Assay Genie Human RBP-4 ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Orbital shaker
  • Aluminum foil
  • Saran Wrap
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Kit Protocol: