Human Resistin PharmaGenie ELISA Kit (SBRS0130)

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Human Resistin PharmaGenie ELISA - Kit Information

Resistin is a 12.5 kDa cysteine-rich hormone secreted by adipose tissue. It is also known as XCP-1 (CEBPE regulated myeloid-specific secreted cysteine-rich protein precursor 1), FIZZ3 (found in inflammatory zone 3), or ADSF (adipocyte-specific secretory factor). The length of the resistin is 108 amino acids in humans, and 114 amino acids in mouse and rat; the molecular weight is ~12.5 kDa. Resistin is an adipokine with physiologic role regarding its involvement with obesity and type II diabetes mellitus (T2DM). Resistin has a high sequence homology among species (43% in a mature protein). Crystal structures of resistin reveal an unusual composition of several subunits that are held together by non-covalent interactions which make up its structure. Each protein subunit comprises a carboxy-terminal disulfide-rich Beta-sandwich "head" domain and an amino-terminal alpha-helical "tail" segment. The globular domain from resistin contains five disulfide bonds. Some studies have shown the important role of resistin linking obesity to T2DM. The underlying belief among those in support of this theory is that serum resistin levels will increase with increased adiposity. Conversely, serum resistin levels have been found to decline with decreased adiposity following medical treatment. This fact takes on significant implications considering the well understood link between central obesity and insulin resistance; marked peculiarities of T2DM. Furthermore, many studies have shown the positive correlations between resistin levels and insulin resistance, and a direct correlation between resistin levels and subjects with T2DM, indicating that such serum resistin increases are accountable for the insulin resistance apparently associated with increased adiposity. In addition to its role in insulin resistance in obese subjects, resistin also plays a role in inflammation and energy homeostasis.

Human Resistin PharmaGenie ELISA - Kit Data

Product SKU:SBRS0130
Application:This kit detects Resistin (90aa). No other active isoforms have been reported. Cross Reactivity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II, NPY and APC.
Gene ID:56729
Gene Names:FIZZ3 / HXCP1 / RETN / RSTN / UNQ407/PRO1199
Synonyms:Resistin (Adipose tissue-specific secretory factor) (ADSF) (C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein) (Cysteine-rich secreted protein A12-alpha-like 2) (Cysteine-rich secreted protein FIZZ3)
Target Species:Human, Mouse, Rat
Compatible Sample Types:Cell Culture Supernatants, Serum
Design Principle:Competition-based
Method of Detection:Colorimetric
Range:0.1-1,000 ng/ml
Sensitivity:446 pg/ml
Recommended Dilution:Human: 2X, Mouse: 2X, Rat: 2X
Storage/Stability:Standard, Biotinylated Resistin peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Kit Components:

1.Pre-Coated 96-well Strip Microplate
2.Wash Buffer
3.Standard Peptide
4.Assay Diluent(s)
5.Biotinylated Peptide
7.TMB One-Step Substrate
8.Stop Solution
9.Assay Diagram
10.Positive Control Sample
11.Capture Antibody
12.Technical Manual

Other materials and equipment required:

The Assay Genie Human Resistin ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Orbital shaker
  • Aluminum foil
  • Saran Wrap
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Kit Protocol: