Human PPIL4 Recombinant Protein (RPPB2156)
- SKU:
- RPPB2156
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q8WUA2
- Research Area:
- Enzymes
Description
| Product Name: | Human PPIL4 Recombinant Protein |
| Product Code: | RPPB2156 |
| Size: | 10µg |
| Species: | Human |
| Target: | PPIL4 |
| Synonyms: | Peptidyl-prolyl cis-trans isomerase-like 4, PPIase, Cyclophilin-like protein PPIL4, Rotamase PPIL4, PPIL4, HDCME13P. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The PPIL solution (0.5 mg/ml) contains 20mM Tris-HCl buffer(pH 8.0), 10% glycerol, 2mM DTT and 0.1M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.�Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).�Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAVLLETTLG DVVIDLYTEE RPRACLNFLK LCKIKYYNYC LIHNVQRDFI IQTGDPTGTG RGGESIFGQL YGDQASFFEA EKVPRIKHKK KGTVSMVNNG SDQHGSQFLI TTGENLDYLD GVHTVFGEVT EGMDIIKKIN ETFVDKDFVP YQDIRINHTV ILDDPFDDPP DLLIPDRSPE PTREQLDSGR IGADEEIDDF KGRSAEEVEE IKAEKEAKTQ AILLEMVGDL PDADIKPPEN VLFVCKLNPV TTDEDLEIIF SRFGPIRSCE VIRDWKTGES LCYAFIEFEK EEDCEKAFFK MDNVLIDDRR IHVDFSQSVA KVKWKGKGGK YTKSDFKEYE KEQDKPPNLV LKDKVKPKQD TKYDLILDEQ AEDSKSSHSH TSKKHKKKTH HCSEEKEDED YMPIKNTNQD IYREMGFGHY EEEESCWEKQ KSEKRDRTQN RSRSRSRERD GHYSNSHKSK YQTDLYERER SKKRDRSRSP KKSKDKEKSK YR |
| Biological Activity: | Specific activity is > 190 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin. |
PPIL4 belongs to an evolutionarily conserved cyclophilin-type PPIase family of proteins. PPIL4, which is ubiquitously expressed with predominant expression in kidney localizes to the nucleus and contains one PPIase cyclophilin-type domain, a lysine-rich domain, a pair of bipartite nuclear targeting sequences and one RRM (RNA recognition motif) domain. The presence of the RRM domain along with nuclear targeting sequences implies that PPIL4 may be involved in transcriptional regulation.
PPIL4 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 512 amino acids (1-492 a.a.) and having a molecular mass of 59.4kDa. The PPIL4 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PPIL4: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Belongs to the cyclophilin-type PPIase family. PPIL4 subfamily. |
| UniProt Protein Details: | Protein type:Isomerase; RNA-binding; EC 5.2.1.8; Cyclophilin Chromosomal Location of Human Ortholog: 6q25.1 Cellular Component: cytoplasm; nucleoplasm Molecular Function:peptidyl-prolyl cis-trans isomerase activity |
| NCBI Summary: | This gene is a member of the cyclophilin family of peptidylprolyl isomerases. The cyclophilins are a highly conserved family, members of which play an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q8WUA2 |
| NCBI GenInfo Identifier: | 74760546 |
| NCBI Gene ID: | 85313 |
| NCBI Accession: | Q8WUA2.1 |
| UniProt Secondary Accession: | Q8WUA2,Q7Z3Q5, B2RD34, |
| UniProt Related Accession: | Q8WUA2 |
| Molecular Weight: | 57,225 Da |
| NCBI Full Name: | Peptidyl-prolyl cis-trans isomerase-like 4 |
| NCBI Synonym Full Names: | peptidylprolyl isomerase like 4 |
| NCBI Official Symbol: | PPIL4�� |
| NCBI Official Synonym Symbols: | HDCME13P�� |
| NCBI Protein Information: | peptidyl-prolyl cis-trans isomerase-like 4 |
| UniProt Protein Name: | Peptidyl-prolyl cis-trans isomerase-like 4 |
| UniProt Synonym Protein Names: | Cyclophilin-like protein PPIL4; Rotamase PPIL4 |
| Protein Family: | Peptidyl-prolyl cis-trans isomerase |
| UniProt Gene Name: | PPIL4�� |
| UniProt Entry Name: | PPIL4_HUMAN |
