Human Thyrotrophic Embryonic Factor Recombinant Protein (CPRT4931)

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CPRT4931
€278

Description

Recombinant Protein DatasheetAntibody MSDS

TEF Human Recombinant Protein

Name:TEF Human
Synonyms:Thyrotroph embryonic factor, TEF, Thyrotroph embryonic factor isoform 1.FBG2, FBS2, FBX6, Fbx6b, F-box only protein 6, F-box protein that recognizes sugar chains 2, F-box/G-domain protein 2, FBXO6.
Introduction:Thyrotroph embryonic factor (TEF) is a nuclear transcription factor which is a part of the bZIP (basic region/leucine zipper) family and PAR subfamily. TEF binds DNA as either a homodimer or heterodimer, and is known to transactivate the TSH beta promoter. TEF accumulates accordingto a robust circadian rhythm and is also inhibits cell growth by down–regulatingbeta chain expression of cytokine receptors.
Description:TEF Human Recombinant produced in E. coli is a single polypeptide chain containing 326 amino acids (1–303) and having a molecular mass of 35.6kDa.TEF is fused to a 23 amino acid His–tag at N–terminus & purified by proprietary chromatographic techniques.
Source:Escherichia Coli.
Pack size:10µg
Physical Appearance:Sterile Filtered clear solution.
Formulation:The TEF solution (0.25mg/ml) contains 20mM Tris–HCl buffer (pH 8.0) and 10% glycerol.
Solubility:N/A
Stability:Store at 4°C if entire vial will be used within 2–4 weeks. Store, frozen at –20°C for longer periods of time. For longterm storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze–thaw cycles.
Purity:Greater than 85% as determined by SDS–PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMSDAGGgKKPPVDPQAgPGPGPGRAAgERGLSGSFPL VLKKLMENPP REARLDKEKgKEKLEEDEAA asTMAVSAS LMPPIWDKTI PYDGESFHLE YMDLDEFLLE NGIPASPTHL AHNLLLPVAE LEGKESASSS TASPPSSSTA IFQPSETVSS TESSLEKERE TPSPIDPNCV EVDVNFNPDP ADLVLSSVPgGELFNPRKHK FAEEDLKPQP MIKKAKKVFV PDEQKDEKYW TRRKKNNVAA KRSRDARRLK ENQITIRAAF LEKENTALRT EVAELRKEVgKCKTIVSKYE TKYGPL.
Biological Activity:N/A
Usage:Assay Genie’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Uniprot:Q10587

TEF Human Recombinant Protein - Protein Information

UniProt Protein Function:TEF: Transcription factor that binds to and transactivates the TSHB promoter. Binds to a minimal DNA-binding sequence 5’- [TC][AG][AG]TTA[TC][AG]-3’. Belongs to the bZIP family. PAR subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: nucleus

Molecular Function:double-stranded DNA binding; protein binding; sequence-specific DNA binding; transcription factor activity

Biological Process: positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; rhythmic process; transcription, DNA-dependent

NCBI Summary:This gene encodes a member of the PAR (proline and acidic amino acid-rich) subfamily of basic region/leucine zipper (bZIP) transcription factors. It is expressed in a broad range of cells and tissues in adult animals, however, during embryonic development, TEF expression appears to be restricted to the developing anterior pituitary gland, coincident with the appearance of thyroid-stimulating hormone, beta (TSHB). Indeed, TEF can bind to, and transactivate the TSHB promoter. It shows homology (in the functional domains) with other members of the PAR-bZIP subfamily of transcription factors, which include albumin D box-binding protein (DBP), human hepatic leukemia factor (HLF) and chicken vitellogenin gene-binding protein (VBP); VBP is considered the chicken homologue of TEF. Different members of the subfamily can readily form heterodimers, and share DNA-binding, and transcriptional regulatory properties. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
UniProt Code:Q10587
NCBI GenInfo Identifier:51338730
NCBI Gene ID:7008
NCBI Accession:Q10587.3
UniProt Secondary Accession:Q10587,Q15729, Q7Z3J7, Q8IU94, Q96TG4, B0QYS8, B2RC22
UniProt Related Accession:Q10587
Molecular Weight:30,635 Da
NCBI Full Name:Thyrotroph embryonic factor
NCBI Synonym Full Names:thyrotrophic embryonic factor
NCBI Official Symbol:TEF  
NCBI Protein Information:thyrotroph embryonic factor
UniProt Protein Name:Thyrotroph embryonic factor
Protein Family:Transcription factor
UniProt Gene Name:TEF  
UniProt Entry Name:TEF_HUMAN
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Additional Information

Product type:
Recombinant Protein
Reactivity:
Human
Host Species:
E.Coli
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