Human TK1 Recombinant Protein (RPPB2667)
- SKU:
- RPPB2667
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P04183
- Research Area:
- Enzymes
Description
| Product Name: | Human TK1 Recombinant Protein |
| Product Code: | RPPB2667 |
| Size: | 20µg |
| Species: | Human |
| Target: | TK1 |
| Synonyms: | Thymidine kinase 1 soluble, thymidine kinase cytosolic, TK2, EC 2.7.1.21. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The TK1 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMSCINL PTVLPGSPSK TRGQIQVILG PMFSGKSTEL MRRVRRFQIA QYKCLVIKYA KDTRYSSSFC THDRNTMEAL PACLLRDVAQ EALGVAVIGI DEGQFFPDIV EFCEAMANAG KTVIVAALDG TFQRKPFGAI LNLVPLAESV VKLTAVCMEC FREAAYTKRL GTEKEVEVIG GADKYHSVCR LCYFKKASGQ PAGPDNKENC PVPGKPGEAV AARKLFAPQQ ILQCSPAN |
Thymidine Kinase 1 (TK1) is a phosphotransferase (a kinase): 2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase. TK1 is present in 2 forms in mammalian cells, TK1 and TK2. Thymidine kinases hold a main function in the synthesis of DNA and thus in cell division, as they are part of the distinctive reaction chain to introduce deoxythymidine (present in the body fluids as a result of degradation of DNA from food and from dead cells) into the DNA. Thymidine kinase is necessary for the action of many antiviral drugs. Thymidine kinase is used to select hybridoma cell lines in production of monoclonal antibodies.
TK1 Human Recombinant produced in E. coli is a single polypeptide chain containing 258 amino acids (1-234) and having a molecular mass of 28.0 kDa.TK1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TK: cytosolic thymidine kinase. Phosphorylated during mitosis. Its enzymatic activity is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells. |
| UniProt Protein Details: | Protein type:EC 2.7.1.21; Xenobiotic Metabolism - drug metabolism - other enzymes; Nucleotide Metabolism - pyrimidine; Kinase, other Chromosomal Location of Human Ortholog: 17q23.2-q25.3 Cellular Component: cytosol Molecular Function:identical protein binding; nucleoside kinase activity; zinc ion binding; thymidine kinase activity; ATP binding Biological Process: response to nutrient levels; skeletal muscle cell proliferation; nucleobase, nucleoside and nucleotide metabolic process; response to toxin; liver development; protein homotetramerization; fetal process involved in parturition; deoxyribonucleoside monophosphate biosynthetic process; response to cortisol stimulus; pyrimidine base metabolic process; gut development; response to copper ion; nucleotide biosynthetic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; thymidine metabolic process; pyrimidine nucleoside salvage; phosphorylation |
| UniProt Code: | P04183 |
| NCBI GenInfo Identifier: | 23503074 |
| NCBI Gene ID: | 7083 |
| NCBI Accession: | P04183.2 |
| UniProt Secondary Accession: | P04183,Q969V0, Q9UMG9, B2RC58, |
| UniProt Related Accession: | P04183 |
| Molecular Weight: | 234 |
| NCBI Full Name: | Thymidine kinase, cytosolic |
| NCBI Synonym Full Names: | thymidine kinase 1, soluble |
| NCBI Official Symbol: | TK1�� |
| NCBI Official Synonym Symbols: | TK2�� |
| NCBI Protein Information: | thymidine kinase, cytosolic; thymidine kinase-1; thymidine kinase 1 soluble isoform |
| UniProt Protein Name: | Thymidine kinase, cytosolic |
| Protein Family: | Thymidine kinase |
| UniProt Gene Name: | TK1�� |
| UniProt Entry Name: | KITH_HUMAN |
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