Human SPP1 Recombinant Protein (RPPB0955)
- SKU:
- RPPB0955
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P10451
- Research Area:
- Growth Factors & Cytokines
Description
| Product Name: | Human SPP1 Recombinant Protein |
| Product Code: | RPPB0955 |
| Size: | 50µg |
| Species: | Human |
| Target: | SPP1 |
| Synonyms: | Secreted Phosphoprotein-1, OPN, BNSP, BSPI, ETA-1, MGC110940, SPP-1, Osteopontin, Bone sialoprotein 1, Urinary stone protein, Nephropontin, Uropontin, SPP1. |
| Source: | HEK293 Cells |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Osteopontin was lyophilized from a 0.2�M filtered solution of 20mM PB and 150mM NaCl, pH 7.2. |
| Solubility: | It is recommended to reconstitute the lyophilized SPP1 in 1xPBS to a concentration no less than 100 �g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | IPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQNAVSSEETNDFKQETLPSKSNESHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPDATDEDITSHMESEELNGAYKAIPVAQDLNAPSDWDSRGKDSYETSQLDDQSAETHSHKQSRLYKRKANDESNEHSDVIDSQELSKVSREFHSHEFHSHEDMLVVDPKSKEEDKHLKFRISHELDSASSEVNVDHHHHHH |
Osteopontin is a glycoprotein that was first identified in osteoblasts and is involved in bone remodeling, immune functions in fibroblasts, macrophages, and lymphocytes during inflammation and wound healing. SPP1 binds tightly to hydroxyapatite. SPP1 forms an integral part of the mineralized matrix. SPP1 is vital to cell-matrix interaction. Secreted Phosphoprotein-1 protects against cardiac ischemia-reperfusion injury via late preconditioning. Expression of both Ostepontin and CD44 in hepatocellular carcinoma is linked with advanced tumor stage and contributes to prognosis information. SPP1 is the most over-expressed gene in intrahepatic cholangiocarcinoma. Secreted Phosphoprotein-1 overexpression is related with interstitial lung diseases.
Osteopontin Human Recombinant is a single, glycosylated, polypeptide chain produced in HEK293 cells, is a full length protein (amino acids 17-314) fused with a polyhistidine tag at the C-terminus, having a total calculated molecular mass of 34.5kDa (The actual molecular mass may be approximately 60-65kDa in SDS-PAGE under reducing conditions due to glycosylation).Osteopontin is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 4q22.1 Cellular Component: extracellular space; cell projection; apical part of cell; perinuclear region of cytoplasm; extracellular region Molecular Function:protein binding; extracellular matrix binding; cytokine activity Biological Process: osteoblast differentiation; extracellular matrix disassembly; neutrophil chemotaxis; extracellular matrix organization and biogenesis; response to vitamin D; biomineral formation; response to steroid hormone stimulus; negative regulation of collateral sprouting of intact axon in response to injury; positive regulation of bone resorption; decidualization; cell adhesion; inflammatory response; embryo implantation |
| NCBI Summary: | The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| UniProt Code: | P10451 |
| NCBI GenInfo Identifier: | 129260 |
| NCBI Gene ID: | 6696 |
| NCBI Accession: | P10451.1 |
| UniProt Secondary Accession: | P10451,Q15681, Q15682, Q15683, Q4W597, Q567T5, Q8NBK2 Q96IZ1, B2RDA1, |
| UniProt Related Accession: | P10451 |
| Molecular Weight: | 33,843 Da |
| NCBI Full Name: | Osteopontin |
| NCBI Synonym Full Names: | secreted phosphoprotein 1 |
| NCBI Official Symbol: | SPP1�� |
| NCBI Official Synonym Symbols: | OPN; BNSP; BSPI; ETA-1�� |
| NCBI Protein Information: | osteopontin; uropontin; nephropontin; SPP1/CALPHA1 fusion; urinary stone protein; early T-lymphocyte activation 1; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) |
| UniProt Protein Name: | Osteopontin |
| UniProt Synonym Protein Names: | Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin |
| Protein Family: | Osteopontin |
| UniProt Gene Name: | SPP1�� |
| UniProt Entry Name: | OSTP_HUMAN |
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