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Human Urocortin PharmaGenie ELISA Kit (SBRS0177)

SKU:
SBRS0177
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
Competitive
€649
Frequently bought together:

Description

Product SKU:SBRS0177
Size:96T
Application:This ELISA kit is designed to detect human, mouse, and rat active Urocortin.
Uniprot:P55089
Gene ID:7349
Gene Names:UCN
Synonyms:Urocortin
Target Species:Human
Compatible Sample Types:Serum
Design Principle:Competition-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Quantitative
Range:0.1-1,000 ng/ml
Sensitivity:1.2 ng/ml
Storage/Stability:Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
  1. Pre-Coated 96-well Strip Microplate
  2. Wash Buffer
  3. Standard Peptide
  4. Assay Diluent(s)
  5. Biotinylated Peptide
  6. HRP-Streptavidin
  7. TMB One-Step Substrate
  8. Stop Solution
  9. Assay Diagram
  10. Positive Control Sample
  11. Capture Antibody
  12. Technical Manual

Other materials and equipment required:

The Assay Genie Human Urocortin PharmaGenie ELISA Kit (SBRS0177) will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Orbital shaker
  • Aluminum foil
  • Saran Wrap
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • SigmaPlot software (or other software that can perform four-parameter logistic regression models)
  1. Prepare all reagents, samples and standards as instructed.
  2. Add 100 ul detection antibody to each well.
  3. Incubate 1.5 h at RT or O/N at 4°C.
  4. Add 100 ul standard or sample to each well.
  5. Incubate 2.5 h at RT.
  6. Add 100 ul prepared streptavidin solution.
  7. Incubate 45 min at RT.
  8. Add 100 ul TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ul Stop Solution to each well.
  11. Read plate at 450 nm immediately.

Urocortin is a member of the sauvagine/corticotropin-releasing factor/urotensin I family. It is an endogenous ligand for corticotropin-releasing factor (CRF) receptors. Urocortin, peptide of 40 amino acids, acts in vitro to stimulate the secretion of adrenocorticotropic hormone (ACTH). It binds with high affinity to CRF receptor types 1, 2-alpha, and 2-beta. Urocortin plays a role in the establishment of normal hearing thresholds. It reduces food intake and regulates ghrelin levels in gastric body and plasma. Urocortin is a potent anorexigenic peptide that induces fed-like motor activity when administered centrally or peripherally in fasted animals. Urocortin is also a potent and long-lasting hypotensive agent and increases coronary blood flow.

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