Human VAMP2 Recombinant Protein (RPPB5101)
- SKU:
- RPPB5101
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P63027
Description
| Product Name: | Human VAMP2 Recombinant Protein |
| Product Code: | RPPB5101 |
| Size: | 20µg |
| Species: | Human |
| Target: | VAMP2 |
| Synonyms: | Vesicle-associated membrane protein 2, SYB2, VAMP-2, Synaptobrevin-2, VAMP2, FLJ11460. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The protein solution (1mg/ml) contains 1X PBS pH 7.4 and 1mM EDTA. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as dtermined by SDS-PAGE. |
| Amino Acid Sequence: | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMSA TAATAPPAAP AGEGGPPAPP PNLTSNRRLQ QTQAQVDEVV DIMRVNVDKV LERDQKLSEL DDRADALQAG ASQFETSAAK LKRKYW |
Synaptobrevin 2 which is an 18 kDa integral membrane protein localized to the cytoplasmic surface of synaptic vesicle, consists of a proline-rich N-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a C-terminal. Synaptobrevin 2 is predominantly expressed in Langerhans islets and glomerular cells. The N-terminal domain of the protein (residues 1-89) forms a specific SNARE complex with the target membrane-associated t- or Q-SNAREs syntaxin 1 and SNAP-25.
VAMP2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 126 amino acids (1-89) and having a molecular mass of 13.8 kDa. The VAMP contains 37 amino acids His-Tag fused at N-terminus and purified by standard chromatography techniques.
| UniProt Protein Function: | VAMP2: a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. Thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Vesicle; Membrane protein, integral Chromosomal Location of Human Ortholog: 17p13.1 Cellular Component: zymogen granule membrane; neuron projection; synaptic vesicle membrane; intracellular membrane-bound organelle; clathrin-coated vesicle; integral to plasma membrane; secretory granule membrane; trans-Golgi network; cytosol; secretory granule; SNARE complex; synaptic vesicle; membrane; perinuclear region of cytoplasm; plasma membrane; synapse; cytoplasmic vesicle; cell junction Molecular Function:calmodulin binding; SNAP receptor activity; protein binding; SNARE binding; protein self-association; syntaxin binding; syntaxin-1 binding; phospholipid binding; calcium-dependent protein binding Biological Process: exocytosis; natural killer cell degranulation; neurotransmitter secretion; neutrophil degranulation; pathogenesis; calcium ion-dependent exocytosis; vesicle fusion; regulation of exocytosis; vesicle-mediated transport; synaptic transmission; protein transport; synaptic vesicle exocytosis; cellular protein metabolic process; glutamate secretion; cellular response to insulin stimulus; Golgi to plasma membrane protein transport; response to glucose stimulus; energy reserve metabolic process; eosinophil degranulation; protein complex assembly; post-Golgi vesicle-mediated transport; regulation of insulin secretion |
| NCBI Summary: | The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P63027 |
| NCBI GenInfo Identifier: | 288558837 |
| NCBI Gene ID: | 6844 |
| NCBI Accession: | P63027.3 |
| UniProt Secondary Accession: | P63027,P19065, Q9BUC2, |
| UniProt Related Accession: | P63027 |
| Molecular Weight: | 13kDa |
| NCBI Full Name: | Vesicle-associated membrane protein 2 |
| NCBI Synonym Full Names: | vesicle-associated membrane protein 2 (synaptobrevin 2) |
| NCBI Official Symbol: | VAMP2�� |
| NCBI Official Synonym Symbols: | SYB2; VAMP-2�� |
| NCBI Protein Information: | vesicle-associated membrane protein 2; synaptobrevin 2; synaptobrevin-2; vesicle associated membrane protein 2 |
| UniProt Protein Name: | Vesicle-associated membrane protein 2 |
| UniProt Synonym Protein Names: | Synaptobrevin-2 |
| Protein Family: | Vesicle-associated membrane protein |
| UniProt Gene Name: | VAMP2�� |
| UniProt Entry Name: | VAMP2_HUMAN |
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