Human Vaspin PharmaGenie ELISA Kit (SBRS0179)
- SKU:
- SBRS0179
- Product Type:
- ELISA Kit
- Reactivity:
- Human
- Applications:
- ELISA
- ELISA Type:
- Competitive
Description
Human Vaspin PharmaGenie ELISA - Kit Information
Visceral adipose tissue-derived serpin (Vaspin), a member of serine protease inhibitor family, is a visceral adipose tissue-derived adipokine with potential antiprotease properties. Vaspin cDNA was isolated by from visceral white adipose tissues (WATs) of Otsuka Long-Evans Tokushima fatty (OLETF) rat, an animal model of abdominal obesity with type 2 diabetes. Human, mouse and rat Vaspin is made up of 395, 394, and 392 amino acids respectively. Vaspin belongs to serine protease inhibitor family and it also shows about 40% homology with 1-antitrypsin. Vaspin ameliorates certain aberrations seen in the diabetic obesity metabolic syndrome by sensitizing insulin action, especially in WATs. Research on Vaspin has been trying for the identification of the potential protease substrate leading to the development of antiprotease inhibitor therapy, which could facilitate the improvement of insulin sensitivity in this metabolic syndrome.
Human Vaspin PharmaGenie ELISA - Kit Data
| Product SKU: | SBRS0179 |
| Size: | 96T |
| Application: | Cross Reactivity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II, NPY and APC. |
| Uniprot: | Q8IW75 |
| Gene ID: | 145264 |
| Gene Names: | SERPINA12 |
| Synonyms: | Serpin A12 (OL-64) (Visceral adipose tissue-derived serine protease inhibitor) (Vaspin) (Visceral adipose-specific serpin) |
| Target Species: | Human, Mouse, Rat |
| Compatible Sample Types: | Cell Culture Supernatants, Serum |
| Design Principle: | Competition-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Range: | 1-10,000 pg/ml |
| Sensitivity: | 26.2 pg/ml |
| Recommended Dilution: | Human: 2X, Mouse: 2X, Rat: 2X |
| Storage/Stability: | Standard, Biotinylated Vaspin peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge. |
Kit Components:
| 1. | Pre-Coated 96-well Strip Microplate |
| 2. | Wash Buffer |
| 3. | Standard Peptide |
| 4. | Assay Diluent(s) |
| 5. | Biotinylated Peptide |
| 6. | HRP-Streptavidin |
| 7. | TMB One-Step Substrate |
| 8. | Stop Solution |
| 9. | Assay Diagram |
| 10. | Positive Control Sample |
| 11. | Capture Antibody |
| 12. | Technical Manual |
Other materials and equipment required:
The Assay Genie Human Vaspin ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.
- Distilled or deionized water
- Precision pipettes to deliver 2 ul to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
