Human Visfatin, His Tag Recombinant Protein (CPRT1092)

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CPRT1092
€278

Description

Recombinant Protein DatasheetAntibody MSDS

Visfatin Human, His Recombinant Protein

Name:Visfatin Human, His
Synonyms:PBEF, Pre-B cell colony-enhancingfactor, Nicotinamide phosphoribosyltransferase NAmPRTase, Nampt, MGC117256, DKFZP666B131, 1110035O14Rik.
Introduction:Excess adiposity is the most important risk in the development of insulin resistance and type 2 diabetes mellitus (T2DM). Adipose tissue produces several proteins (adipocytokines) such as leptin, adiponectin, resistin, tumor necrosis factor–?, and IL–6, that modulate insulin sensitivity and appear to play an important role in the pathogenesis of insulin resistance, diabetes, dyslipidemia, inflammation, and atherosclerosis. However, the mechanisms by which fat tissue induces insulin resistance and the role of adipocytokines in the pathogenesis of T2DM have not been well established.Visfatin, also known as pre–B cell colony–enhancingfactor (PBEF), is a cytokine that is highly expressed in visceral fat and was originally isolated as a secreted factor that synergizes with IL–7 and stem cell factors to promote the growth of B cell precursors. Visfatin homologs have been identified in carp, invertebrate mollusks, and bacteria, as well as in vertebrates, includinghumans and the mouse. It has been postulated to play a role in innate immunity.Visfatin exerts insulin–mimetic effects that are dose–dependent and quantitatively similar to those of insulin in stimulatingmuscle and adipocyte glucose transport, and in inhibitinghepatocyte glucose production. Intravenous injection of recombinant visfatin in mice decreased plasma glucose in a dose–dependent fashion. In keepingwith its insulin–mimetic effects, visfatin was as effective as insulin in reducinghyperglycemia in insulin–deficient diabetic mice. Visfatin was also found to be bound to and activate insulin receptor, causingreceptor phosphorylation and the activation of downstream signalingmolecules. However, visfatin and insulin did not compete for bindingto the insulin receptor, indicatingthat the two proteins were recognized by different regions of the receptor. Thus, visfatin might play a role in glucose homeostasis and dysregulation in biosynthesis or signal transduction, and might contribute to the pathogenesis of diabetes.
Description:Visfatin Human Recombinant produced in E.Coli is a single, non–glycosylated, polypeptide chain containing 511 amino acids and having a molecular mass of 57 kDa. The recombinant human Visfatin is fused to His tag at N–Terminus.
Source:Escherichia Coli.
Pack size:25µg
Physical Appearance:Sterile Filtered solution at a concentration of 1mg/ml.
Formulation:Visfatin His tagprotein contains 20mM Tris pH–8, 0.1mM DTT & 10% glycerol.
Solubility:N/A
Stability:Store at 4°C if entire vial will be used within 2–4 weeks. Store, frozen at –20°C for longer periods of time. Please avoid freeze thaw cycles.
Purity:Greater than 95.0% as determined by(a) Analysis by RP–HPLC.(b) Analysis by SDS–PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MNPAAEAEFN ILLATDSYKV THYKQYPPNT SKVYSYFECR EKKTENSKLR KVKYEETVFY GLQYILNKYL KGKVVTKEKI QEAKDVYKEH FQDDVFNEKgWNYILEKYDgHLPIEIKAVP EGFVIPRGNV LFTVENTDPE CYWLTNWIET ILVQSWYPIT VATNSREQKK ILAKYLLETS GNLDGLEYKL HDFGYRGVSS QETAGIGASA HLVNFKGTDT VAGLALIKKY YGTKDPVPGY SVPAAEHSTI TAWGKDHEKD AFEHIVTQFS SVPVSVVSDS YDIYNACEKI WGEDLRHLIV SRSTQAPLII RPDSGNPLDT VLKVLEILGK KFPVTENSKgYKLLPPYLRV IQGDGVDINT LQEIVEGMKQ KMWSIENIAF GSGGGLLQKL TRDLLNCSFK CSYVVTNGLgINVFKDPVAD PNKRSKKGRL SLHRTPAGNF VTLEEGKGDL EEYGQDLLHT VFKNGKVTKS YSFDEIRKNA QLNIELEAAH H.
Biological Activity:N/A
Usage:Assay Genie’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Uniprot:P43490

Visfatin Human, His Recombinant Protein - Protein Information

UniProt Protein Function:PBEF: an ubiquitous protein originally described as a cytokine which acts on early B-lineage precursor cells. Other reports indicate that PBEF is not a cytokine-like secreted protein but an intracellular protein associated with the cell cycle. Contains a catalytically active nicotinate phosphoribosyltransferase domain, an enzyme involved in nicotinamide adenine dinucleotide (NAD) biosynthesis.
UniProt Protein Details:

Protein type:Cell cycle regulation; Transferase; EC 2.4.2.12; Cytokine; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide

Chromosomal Location of Human Ortholog: 7q22.3

Cellular Component: extracellular space; cytosol; nucleus

Molecular Function:protein binding; protein homodimerization activity; cytokine activity; nicotinate-nucleotide diphosphorylase (carboxylating) activity; drug binding; nicotinamide phosphoribosyltransferase activity

Biological Process: circadian rhythm; vitamin metabolic process; positive regulation of smooth muscle cell proliferation; female pregnancy; signal transduction; response to organic cyclic substance; NAD biosynthetic process; cell-cell signaling; NAD metabolic process; nicotinamide metabolic process; positive regulation of cell proliferation; insulin receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; water-soluble vitamin metabolic process; positive regulation of nitric-oxide synthase biosynthetic process

NCBI Summary:This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011]
UniProt Code:P43490
NCBI GenInfo Identifier:1172027
NCBI Gene ID:10135
NCBI Accession:P43490.1
UniProt Related Accession:P43490
Molecular Weight:
NCBI Full Name:Nicotinamide phosphoribosyltransferase
NCBI Synonym Full Names:nicotinamide phosphoribosyltransferase
NCBI Official Symbol:NAMPT  
NCBI Official Synonym Symbols:VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik  
NCBI Protein Information:nicotinamide phosphoribosyltransferase
UniProt Protein Name:Nicotinamide phosphoribosyltransferase
UniProt Synonym Protein Names:Pre-B-cell colony-enhancing factor 1; Pre-B cell-enhancing factor; Visfatin
UniProt Gene Name:NAMPT  
UniProt Entry Name:NAMPT_HUMAN
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Additional Information

Product type:
Recombinant Protein
Reactivity:
Human
Host Species:
E.Coli
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