Indoleamine 2,3-Dioxygenase 1 (IDO1) Inhibitor Screening Kit (BN01134)

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BN01134
  • Indoleamine 2,3-Dioxygenase 1 (IDO1) Inhibitor Screening Kit (BN01134)
  • Indoleamine 2,3-Dioxygenase 1 (IDO1) Inhibitor Screening Kit (BN01134)
€822

Description

ELISA Kit Technical ManualMSDS

Indoleamine 2,3-Dioxygenase 1 (IDO1) Inhibitor Screening Kit

Indoleamine 2,3-Dioxygenase 1 (IDO1, EC 1.13.11.52) is a cytoplasmic hemoprotein that oxidizes tryptophan yielding N-formylkynurenine (NFK). In mammals, this reaction is the first and rate limiting step in the kynurenine catabolic pathway. IDO1 activity is low under normal physiological conditions, but is dramatically upregulated by proinflammatory cytokines such as interferon- This short-term activation of IDO1 occurs as part of the innate immune response and helps to inhibit the growth of pathogens and parasites. IDO1 activation can also promote host immune tolerance by exerting an immunosuppressive effect. IDO1 expression by tumor cells plays a substantial role in tumor immune tolerance, aiding tumors in evading detection and destruction. Chronic induction of IDO1 expression has been found in patients with many different types of cancer and increased IDO1 activity is correlated with more extensive disease spread and worsening prognosis. IDO1 has thus become an attractive pharmacological target for development of novel antineoplastics and adjuvants to increase the efficacy of conventional chemotherapy. Assay Genie's IDO1 Inhibitor Screening Kit enables rapid screening of test compounds for modulation of IDO1 activity. Unlike conventional colorimetric IDO1 assays based upon UV absorbance, the assay uses a fluorogenic developer that selectively reacts with NFK to produce a highly fluorescent product detectable in the visible range (Ex/Em = 402/488 nm). This ensures a high signal-to-background ratio and little interference due to short wavelength absorbance by test compounds. The assay is highly sensitive, has a simple no-wash protocol and is high-throughput adaptable. The kit contains a complete set of reagents sufficient for performing 100 reactions in a 96-well plate format.

Figure: (a) Measurement of IDO1 inhibition in presence and absence of 1 μM of IDO5L (a potent, highly selective competitive IDO1 inhibitor) and 100 μM of norharmane (a natural product that acts as a weak IDO1 inhibitor). The no inhibitor reaction contained assay buffer with 0.1% DMSO (v/v) as a solvent control. (b) Dose-response curve for IDO1 inhibition by the included selective IDO1 Inhibitor IDO5L. The IC 50 value (24.2 ± 1.3 nM) was derived by 4-parameter logistic curve fitting with each point representing the mean ± SEM of at least 3 replicates. All assays were performed according to the kit protocol.

Key Information Description

Product SKU

BN01134

Size

100 assays

Detection Method

Fluorescence (Ex/Em 402/488 nm)

Species Reactivity

Mammalian

Applications

  • Rapid, high-throughput screening of drugs and novel ligands.
  • Development of structure-activity relationship (SAR) models to predict IDO1 inhibition liability of novel compounds.

Features and Benefits

  • Simple, highly sensitive, high-throughput compatible
  • Rapid screening of IDO1 inhibitors or ligands
  • Kit includes the IDO1 inhibitor IDO5L and a stable, recombinant human IDO1 Enzyme

Kit Components

  • IDO1 Assay Buffer
  • Antioxidant Mix (100X)
  • IDO1 Substrate (L-tryptophan)
  • IDO Inhibitor (IDO5L)
  • Fluorogenic Developer Solution
  • Recombinant Human IDO1
  • Microplate Sealing Film

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans

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Additional Information

Product type:
Assay
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