Intestinal Permeability Assay Kit
INTESTINAL PERMEABILITY is a phenomenon of the gut wall in which leakage of molecules and ions below 0.4 nm occurs from the gut lumen into blood circulation. This paracellular leakage occurs through tight junctions between epithelial cells. Elevated paracellular leakage has been implicated in many disorders including type 1 and type 2 diabetes, obesity, inflammatory bowel disease, celiac disease, Parkinson's disease and cancer.Assay Genie's intestinal permeability assay kit is based on measuring the ratio of the absorption of two non-metabolized sugars to through the intestines. Test subjects drink a prescribed amount of lactulose and mannitol and the % absorption of these sugars is determined by the amount of excreted lactulose and mannitol measured during the first 6 hours after ingestion. The degree of intestinal permeability is reflected by the ratio of the % absorption of lactulose to % absorption of mannitol. An increase in this ratio indicates increased intestinal permeability since lactulose is only absorbed though intercellular spaces. Lactulose and mannitol are measured in separate assays using the included Lactulose Assay Kit and Mannitol Assay Kit, respectively.
ApplicationsDetermination of intestinal permeability (leaky gut syndrome) through measuring lactulose/mannitol ratio in urine samples.
- Simple and convenient. Both assays require addition of single working reagent and can be completed within 60 minutes. Both assays are performed at room temperature. No 37°C heater is needed.
- High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Intestinal Permeability Assay Kit Information:
|Kit Includes:||LACTULOSE ASSAY KIT (BA0128) Assay Buffer: 6 mL Standard: 400 uL 15 mM Lactulose Enzyme A: Dried Enzyme B: 120 uL Enzyme Buffer: 150 uL PMS Solution: 1.5 mL D-MANNITOL ASSAY KIT (BA0133) Assay Buffer: 10 mL Standard: 0.5 mL 20 mM D-Mannitol Enzyme: 120 uL|
|Kit Requires:||Pipetting devices, centrifuge tubes, clear flat-bottom 96-well plates, and plate or cuvette reader. Use your own discretion when choosing appropriate materials for this test..|
|Method of Detection:||OD565nm|
|Detection Limit:||D-Mannitol: 7 uM; Lactulose: 3 uM|
|Protocol Length:||60 and 30 min|
|Storage:||Store all components at -20°C upon receiving|
|Shelf Life:||6 months|