JNK Activity Assay Kit (BN00676)

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SKU:
BN00676
€756

Description

ELISA Kit Technical ManualMSDS

JNK Activity Assay Kit

c-Jun N-terminal kinase (JNK) is one of the several main MAP kinase groups identified in mammals. Recent evidences suggest that activation of JNK may play an important role in neuronal apoptosis and other physiological and pathological processes. The JNK Activity Immunoassay Kit utilizes a JNK-specific antibody to immunoprecipitate JNK from cell lysate. Activity of the JNK is then determined in a kinase reaction using recombinant c-Jun as substrate. Phosphorylation of the c-Jun can be analyzed by Western blot analysis using a phospho-c-Jun specific antibody. The kit specifically detects JNK, other kinase activity would not be detected.

Key Information Description

Product SKU

BN00676

Size

40 assays

Detection Method

Western blot analysis using a phospho-c-Jun specific antibody at 1:1000 dilutions

Species Reactivity

Mammalian

Applications

The JNK Activity Immunoassay Kit utilizes a JNK-specific antibody to immunoprecipitate JNK from cell lysate. JNK-specific activity is then analyzed by determining the phosphorylation of c-Jun by Western blotting using a phospho-c-Jun specific antibody.

Features and Benefits

  • Simple procedure
  • The kit is suitable for both research and drug discovery
  • The assay utilizes a N-terminal c-Jun fusion protein bead to selectively "pull down" JNK from cell lysate c-Jun phosphorylation is then measured using a phospho-c-Jun specific antibody.

Kit Components

  • Kinase Extraction Buffer
  • JNK Specific Antibody
  • Protein A Sepharose
  • c-Jun Protein/ATP Mixture
  • Kinase Assay Buffer
  • Phospho-cJun Specific Antibody

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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