Anti-CASP9 Antibody (CAB18676)

Antibody Name:	Anti-CASP9 Antibody [KO Validated]
Antibody SKU:	CAB18676
Antibody Size:	20uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthesized peptide derived from human CASP9.

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, HepG2, Mouse brain, Rat brain

Immunogen:	A synthesized peptide derived from human CASP9.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	842
Uniprot:	P55211
Cellular Location:
Calculated MW:	17kDa/30kDa/36kDa/46kDa
Observed MW:	43kDa/45kDa

	Western blot analysis of extracts of various cell lines, using CASP9 antibody (CAB18676) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Western blot analysis of extracts from normal (control) and CASP9 knockout (KO) 293T cells, using CASP9 antibody (CAB18676) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of C6 cells using [KO Validated] CASP9 Rabbit mAb (CAB18676) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] CASP9 Rabbit mAb (CAB18676) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Synonyms:

CASP9, APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56, caspase-9

Background:

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	CASP9: a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two isoforms.
UniProt Protein Details:	Protein type:Apoptosis; EC 3.4.22.62; Protease Chromosomal Location of Human Ortholog: 1p36.21 Cellular Component: apoptosome; cytosol Molecular Function:cysteine-type endopeptidase activity; enzyme activator activity; peptidase activity; protein binding; protein kinase binding; SH3 domain binding Biological Process: apoptosis; caspase activation via cytochrome c; DNA damage response, signal transduction; DNA damage response, signal transduction resulting in induction of apoptosis; platelet formation; response to DNA damage stimulus
NCBI Summary:	This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]
UniProt Code:	P55211
NCBI GenInfo Identifier:	28558771
NCBI Gene ID:	842
NCBI Accession:	P55211.3
UniProt Secondary Accession:	P55211,O95348, Q53Y70, Q5JRU9, Q5UGI1, Q92852, Q9BQ62 Q9UEQ3, Q9UIJ8, B4E1A3,
UniProt Related Accession:	P55211
Molecular Weight:
NCBI Full Name:	Caspase-9
NCBI Synonym Full Names:	caspase 9
NCBI Official Symbol:	CASP9
NCBI Official Synonym Symbols:	MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
NCBI Protein Information:	caspase-9
UniProt Protein Name:	Caspase-9
UniProt Synonym Protein Names:	Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3
Protein Family:	Caspase
UniProt Gene Name:	CASP9

Antibodies	ELISA Kits
Anti-Caspase-9 Antibody (CAB0019)	Human Caspase-9 ELISA Kit
Anti-Caspase-9 Antibody (CAB0281)[KO Validated]	Human Cleaved CASP9 (Cleaved Caspase-9) ELISA Kit (HUFI04740)
Anti-Caspase-9 Mouse Monoclonal Antibody (CAB10097)	Human CASP 9 (Caspase 9) ELISA Kit (HUES01822)
Anti-Caspase-9 Antibody (CAB11451)	Human Caspase-9 (CASP9) ELISA Kit
Anti-Caspase-9 Antibody (CAB2636)	Caspase 9 Colorimetric Cell-Based ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Mouse IgG (H+L) Antibody (CABS003)