L-Carnitine Colorimetric/Fluorometric Assay Kit (BN00866)

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SKU:
BN00866
  • L-Carnitine Colorimetric/Fluorometric Assay Kit (BN00866)
  • L-Carnitine Colorimetric/Fluorometric Assay Kit (BN00866)
€684

Description

ELISA Kit Technical ManualMSDS

L-Carnitine Colorimetric/Fluorometric Assay Kit

Carnitine is a quaternary ammonium compound biosynthesized from the amino acids lysine and methionine. It is required for transport of fatty acids into the mitochondrial matrix via the carnitine/acylcarnitine shuttle where Beta-oxidation occurs, acetate is generated and the acetate utilized in the TCA cycle for the generation of energy. L-Carnitine is often sold as a nutritional supplement. Carnitine exists in two stereoisomers. Only L-carnitine is biologically active. Assay Genie's L-Carnitine Assay Kit is a simple convenient means of measuring free L-Carnitine in biological samples such as serum. The assay transfers an acetyl group from CoA to carnitine and the free CoA formed is further processed with subsequent oxidation of the Oxi-Red probe to give fluorescence (Ex/Em 535 nm 587 nm) and absorbance (570 nm). The normal range for serum L-Carnitine is ~20-100 µM. The detection sensitivity is ~1 µM for the fluorometric assay and ~10 µM for the colorimetric assay.

Figure 1: Standard Graphs obtained from following Kit Protocol.

Key Information Description

Product SKU

BN00866

Size

100 assays

Detection Method

Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)

Species Reactivity

Mammalian

Applications

The detection sensitivity is ~1 µM for the fluorometric assay and ~10 µM for the colorimetric assay.

Features and Benefits

  • Simple procedure; takes ~ 30 minutes
  • Fast and convenient
  • Kit contains all necessary reagents for accurate measurement of L-Carnitine levels

Kit Components

  • Carnitine Assay Buffer
  • Carnitine Probe (in DMSO)
  • Carnitine Converting Enzyme
  • Carnitine Substrate Mix
  • Carnitine Development Mix
  • Carnitine Standard (10 µmol)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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