Anti-LAMC2 Antibody (CAB1869)

Antibody Name:	Anti-LAMC2 Antibody
Antibody SKU:	CAB1869
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 862-1111 of human LAMC2 (NP_061486.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 862-1111 of human LAMC2 (NP_061486.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SDQS FQVE EAKR IKQK ADSL SSLV TRHM DEFK RTQK NLGN WKEE AQQL LQNG KSGR EKSD QLLS RANL AKSR AQEA LSMG NATF YEVE SILK NLRE FDLQ VDNR KAEA EEAM KRLS YISQ KVSD ASDK TQQA ERAL GSAA ADAQ RAKN GAGE ALEI SSEI EQEI GSLN LEAN VTAD GALA MEKG LASL KSEM REVE GELE RKEL EFDT NMDA VQMV ITEA QKVD TRAK NAGV TIQD TLNT LDGL LHLM GM
Gene ID:	3918
Uniprot:	Q13753
Cellular Location:	Secreted, basement membrane, extracellular matrix, extracellular space
Calculated MW:	121kDa/130kDa
Observed MW:	131kDa

Synonyms:

LAMC2, B2T, BM600, CSF, EBR2, EBR2A, LAMB2T, LAMNB2

Background:

This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two transcript variants resulting from alternative splicing of the 3' terminal exon, and encoding different isoforms of gamma 2 chain, have been described. The two variants are differentially expressed in embryonic tissues, however, the biological significance of the two forms is not known. Transcript variants utilizing alternative polyA_signal have also been noted in literature.

UniProt Protein Function:	LAMC2: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Ladsin exerts cell- scattering activity toward a wide variety of cells, including epithelial, endothelial, and fibroblastic cells. Defects in LAMC2 are a cause of epidermolysis bullosa junctional Herlitz type (H-JEB); also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB defines a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement membrane. H-JEB is a severe, infantile and lethal form. Death occurs usually within the first six months of life. Occasionally, children survive to teens. H-JEB is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Secreted; Extracellular matrix; Motility/polarity/chemotaxis; Secreted, signal peptide Chromosomal Location of Human Ortholog: 1q25-q31 Cellular Component: extracellular space; laminin-5 complex; laminin-2 complex; membrane; perinuclear region of cytoplasm; extracellular region; cell cortex Molecular Function:heparin binding Biological Process: extracellular matrix disassembly; hemidesmosome assembly; epidermis development; extracellular matrix organization and biogenesis; cell adhesion Disease: Epidermolysis Bullosa, Junctional, Non-herlitz Type; Epidermolysis Bullosa, Junctional, Herlitz Type
NCBI Summary:	Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two transcript variants resulting from alternative splicing of the 3' terminal exon, and encoding different isoforms of gamma 2 chain, have been described. The two variants are differentially expressed in embryonic tissues, however, the biological significance of the two forms is not known. Transcript variants utilizing alternative polyA_signal have also been noted in literature. [provided by RefSeq, Aug 2011]
UniProt Code:	Q13753
NCBI GenInfo Identifier:	90185107
NCBI Gene ID:	3918
NCBI Accession:	Q13753.2
UniProt Secondary Accession:	Q13753,Q02536, Q02537, Q13752, Q14941, Q14DF7, Q2M1N2 Q5VYE8,
UniProt Related Accession:	Q13753
Molecular Weight:
NCBI Full Name:	Laminin subunit gamma-2
NCBI Synonym Full Names:	laminin, gamma 2
NCBI Official Symbol:	LAMC2
NCBI Official Synonym Symbols:	B2T; CSF; EBR2; BM600; EBR2A; LAMB2T; LAMNB2
NCBI Protein Information:	laminin subunit gamma-2; BM600-100kDa; laminin B2t chain; CSF 140 kDa subunit; nicein subunit gamma; kalinin subunit gamma; ladsin 140 kDa subunit; epiligrin subunit gamma; cell-scattering factor 140 kDa subunit; large adhesive scatter factor 140 kDa subunit
UniProt Protein Name:	Laminin subunit gamma-2
UniProt Synonym Protein Names:	Cell-scattering factor 140 kDa subunit; CSF 140 kDa subunit; Epiligrin subunit gamma; Kalinin subunit gamma; Kalinin/nicein/epiligrin 100 kDa subunit; Ladsin 140 kDa subunit; Laminin B2t chain; Laminin-5 subunit gamma; Large adhesive scatter factor 140 kDa subunit; Nicein subunit gamma
Protein Family:	Laminin
UniProt Gene Name:	LAMC2
UniProt Entry Name:	LAMC2_HUMAN

	Western blot analysis of extracts of rat lung, using LAMC2 antibody (CAB1869) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunofluorescence analysis of A431 cells using LAMC2 Polyclonal Antibody (CAB1869) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of rat kidney using LAMC2 Polyclonal Antibody (CAB1869) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of mouse kidney using LAMC2 Polyclonal Antibody (CAB1869) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.