Leucine Aminopeptidase (LAP) Activity Assay Kit (Fluorometric) (BN00765)

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BN00765
  • Leucine Aminopeptidase (LAP) Activity Assay Kit (Fluorometric) (BN00765)
  • Leucine Aminopeptidase (LAP) Activity Assay Kit (Fluorometric) (BN00765)
  • Leucine Aminopeptidase (LAP) Activity Assay Kit (Fluorometric) (BN00765)
$668.36

Description

ELISA Kit Technical ManualMSDS

Leucine Aminopeptidase (LAP) Activity Assay Kit (Fluorometric)

Leucine aminopeptidases (EC 3.4.11.1) (LAPs) are a diverse set of exopeptidases that catalyze the hydrolysis of leucine residues from the amino-termini of proteins or peptides. LAPs are ubiquitous enzymes present among animals, plants and prokaryotes. Previously, they were thought to typically play important roles in cell maintenance, growth and development. However, research in the recent years has identified multiple secondary functions for these enzymes in animals and microbes including transcriptional regulation and vesicle transport. Studies have implicated LAP enzymes in tumor cell proliferation, invasion and angiogenesis. Placental LAP is used as a biomarker in ovarian epithelial cancer while adipocyte-derived LAP is used as a marker of endometrial cancer cell proliferation and differentiation. LAP enzymes are also known to be involved in catabolism of oxytocin and vasopressin and insulin regulation of GLUT4 receptors in diabetes. Assay Genie's Leucine Aminopeptidase assay kit provides a quick, sensitive and easy way for measuring total LAP activity in various samples. In this assay, LAPs hydrolyze leucine from the fluorescent probe and the amount of fluorescent probe detected at Ex/Em 368/460 nm is used to determine the total activity of the LAP enzymes. The assay is simple to perform, high-throughput adaptable and can detect less than 0.1 mU of LAP activity

Figure: (a) AMC standard curve; (b) Reaction kinetics of leucine aminopeptidase activity in rat liver (6.6 µg protein) and Jurkat cells (8 µg protein) using appropriate background controls; (c) Leucine aminopeptidase specific activity was calculated in rat liver and Jurkat cell lysates. Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00765

Size

100 assays

Kit Summary

  • Detection method - Fluorescence (Ex/Em = 368/460 nm)
  • Applications- Detection of LAP activity in mammalian tissues, cell culture and purified enzyme

Detection Method

Fluorometric (Ex/Em = 368/460 nm)

Species Reactivity

Mammalian

Applications

Detection of LAP activity in mammalian tissues, cell culture and purified enzyme

Features and Benefits

  • Simple one-step reaction
  • Takes only 1-2 hrs
  • Non-radiometric fluorescent detection
  • HTP adaptable

Kit Components

  • LAP Assay Buffer
  • LAP Substrate
  • AMC-Standard (1 mM)
  • LAP Positive Control

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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