Anti-LIG1 Antibody (CAB1858)

Antibody Name:	Anti-LIG1 Antibody
Antibody SKU:	CAB1858
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 565-790 of human LIG1 (NP_000225.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	293T, K-562

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 565-790 of human LIG1 (NP_000225.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	CEYK YDGQ RAQI HALE GGEV KIFS RNQE DNTG KYPD IISR IPKI KLPS VTSF ILDT EAVA WDRE KKQI QPFQ VLTT RKRK EVDA SEIQ VQVC LYAF DLIY LNGE SLVR EPLS RRRQ LLRE NFVE TEGE FVFA TSLD TKDI EQIA EFLE QSVK DSCE GLMV KTLD VDAT YEIA KRSH NWLK LKKD YLDG VGDT LDLV VIGA YLGR GKRA GRYG GFLL ASYD EDSE EL
Gene ID:	3978
Uniprot:	P18858
Cellular Location:	Nucleus
Calculated MW:	88kDa/98kDa/101kDa
Observed MW:	140KDa

Synonyms:

LIG1

Background:

This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	LIG1: is an enzyme that functions in DNA replication and the base excision repair process. Mutations leading to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents.
UniProt Protein Details:	Protein type:Ligase; EC 6.5.1.1; DNA repair, damage Chromosomal Location of Human Ortholog: 19q13.2-q13.3 Cellular Component: nucleoplasm; Golgi apparatus; intracellular membrane-bound organelle; mitochondrion; chromosome; nucleus Molecular Function:DNA binding; DNA ligase activity; metal ion binding; DNA ligase (ATP) activity; ATP binding Biological Process: anatomical structure morphogenesis; mismatch repair; V(D)J recombination; DNA strand elongation during DNA replication; DNA repair; lagging strand elongation; double-strand break repair via homologous recombination; double-strand break repair via nonhomologous end joining; telomere maintenance via semi-conservative replication; response to hydrogen peroxide; base-excision repair; nucleotide-excision repair; cell division; transcription-coupled nucleotide-excision repair; double-strand break repair; telomere maintenance via recombination; DNA ligation during DNA repair; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; telomere maintenance; DNA metabolic process
NCBI Summary:	This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
UniProt Code:	P18858
NCBI GenInfo Identifier:	118773
NCBI Gene ID:	3978
NCBI Accession:	P18858.1
UniProt Related Accession:	P18858
Molecular Weight:	Predicted Band Size: 102kDa
NCBI Full Name:	DNA ligase 1
NCBI Synonym Full Names:	DNA ligase 1
NCBI Official Symbol:	LIG1
NCBI Protein Information:	DNA ligase 1
UniProt Protein Name:	DNA ligase 1
UniProt Synonym Protein Names:	DNA ligase I; Polydeoxyribonucleotide synthase [ATP] 1
UniProt Gene Name:	LIG1
UniProt Entry Name:	DNLI1_HUMAN

	Western blot analysis of extracts of various cell lines, using LIG1 Rabbit pAb (CAB1858) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of MCF-7 cells using LIG1 antibody (CAB1858). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of A549 cells using LIG1 antibody (CAB1858).

Antibodies	ELISA Kits
LIG1 Antibody (PACO00713)	Human DNA ligase 1 (LIG1) ELISA Kit
LIG1 Antibody (PACO10248)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)