Lipase Activity Colorimetric Assay Kit II (BN00942)

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BN00942
  • Lipase Activity Colorimetric Assay Kit II (BN00942)
  • Lipase Activity Colorimetric Assay Kit II (BN00942)
€619

Description

ELISA Kit Technical ManualMSDS

Lipase Activity Colorimetric Assay Kit II  

Lipase is a subclass of the esterases that catalyze the hydrolysis of ester bonds in water-insoluble, lipid substrates. Lipases perform essential roles in the digestion, transport and processing of dietary lipids (e.g. triglycerides, fats, oils) in most, if not all, living organisms. In humans, pancreatic lipases are the key enzyme responsible for breaking down fats in the digestive system by converting triglycerides to monoglycerides and free fatty acids. During the damage of the pancreas, lipase levels can rise 5 to 10-fold within 24 to 48 hours. The kit provides a simple, sensitive, and reliable assay for rapid analysis of Lipase in samples. In the assay, Lipases hydrolyze a specific substrate to generate a product which reacts with the DTNB probe to generate color (412 nm). The kit is also suitable for high throughput analyses.

Figure: (a) Lipase Activity Standard Curve obtained using Kit Protocol (b) Assays Kinetics

Key Information Description

Product SKU

BN00942

Size

100 assays

Detection Method

Absorbance (412 nm)

Species Reactivity

Mammalian

Applications

N/A

Features and Benefits

  • Simple procedure; takes ~ 60-90 minutes
  • Fast and convenient
  • Sensitive assays for measuring Lipase activity in various biological samples and the kit is also suitable for high throughput analyses.

Kit Components

  • Lipase Assay Buffer
  • DTNB Probe
  • Lipase Substrate
  • TNB Standard (5 mM)
  • Lipase Positive Control

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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