Live/Dead Cell Viability Assay Kit (Mammalian Cell) (BN00733)

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BN00733
£496.32

Description

ELISA Kit Technical ManualMSDS

Live/Dead Cell Viability Assay Kit (Mammalian Cell)

Quantification of number of live and dead cells is an indispensable tool in cell biology research. Assay Genie's Live/Dead Cell Viability Assay Kit, provides a two-color fluorescence method that is based on the simultaneous determination of live and dead cells using two different dyes. Live cell dye easily penetrates intact,Live cells and intracellular esterase hydrolyzes the dye to produce a hydrophilic, strongly fluorescent compound that is retained in the cell cytoplasm which can be measured at Ex/Em = 485/530 nm. Dead cell dye enters damaged cell membranes and undergoes a 40-fold enhancement of fluorescence upon binding to nucleic acid, thereby producing a bright red fluorescence (Ex/Em = 495/635 nm) in dead cells. This assay kit provides an easy-to-use, non-radioactive, histological and FACS-based method for measuring cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis.

Figure 1 : Analysis of Live/Dead HeLa Cells by Microscopy: HeLa cells were cultured overnight with (d-f) or without (a-c)
comptothecin (5 µM), which induces apoptosis. Next day, cells were treated with Staining Solution, as described in the protocol. Light and fluorescence images of cells were taken using Nikon TiE microscope. Treatment with comptothecin caused increased apoptosis in cells, which is demonstrated by increased number of dead cells, as shown below.

Figure 2: Analysis of Live/Dead Jurkat Cells by Flow Cytometry: Jurkat cells (10 6 cells/ml) were grown in RPMI media supplemented with 10% FBS. Cells were treated with or without camptothecin (5 µM) overnight. Next day, cells were stained with
Staining Solution, as described in the protocol. The graph (right side) displays the cytotoxic effect of the compound, illustrating
apoptosis using Dead (Red) and Live (Green) Cell Staining Dye. In control cells (No Treatment), majority of the cells were sorted in FL-1 Channel (Green) and few were in FL-3 (Red) channel. In treated cells, majority of the cells were sorted in FL-3 Channel (Red) and some cells were in FL-1 (Green) channel.

Key Information Description

Product SKU

BN00733

Size

100 assays

Detection Method

Fluorescent microscopy or FACS analysis (Ex/Em 485/530 nm) to detect stained live cells; (Ex/Em 495/635) to detect stained dead cells

Species Reactivity

Mammalian

Applications

Screening/studying/characterization of stimulators/inhibitors that affect cell viability.

Features and Benefits

  • Easy to use ProtocolNon-radioactive detection.
  • Detect using either microscopy or FACS.Measure Cell Proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis

Kit Components

  • Assay Buffer
  • Live Cell Staining Dye
  • Dead Cell Staining Dye

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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