Anti-LTF Antibody (CAB12902)

Antibody Name:	Anti-LTF Antibody
Antibody SKU:	CAB12902
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 500-710 of human LTF (NP_002334.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse lung, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 500-710 of human LTF (NP_002334.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GSCK FDEY FSQS CAPG SDPR SNLC ALCI GDEQ GENK CVPN SNER YYGY TGAF RCLA ENAG DVAF VKDV TVLQ NTDG NNNE AWAK DLKL ADFA LLCL DGKR KPVT EARS CHLA MAPN HAVV SRMD KVER LKQV LLHQ QAKF GRNG SDCP DKFC LFQS ETKN LLFN DNTE CLAR LHGK TTYE KYLG PQYV AGIT NLKK CSTS PLLE ACEF LRK
Gene ID:	4057
Uniprot:	P02788
Cellular Location:	Cytoplasm, Cytoplasmic granule, Nucleus, Secreted
Calculated MW:	73kDa/78kDa
Observed MW:	78kDa

Synonyms:

LTF, GIG12, HEL110, HLF2, LF

Background:

This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Antimicrobial, antiviral, antifungal and antiparasitic activity has been found for this protein and its peptides. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	LTF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative promoter.
UniProt Protein Details:	Protein type:Secreted; Protease; EC 3.4.21.-; Secreted, signal peptide Chromosomal Location of Human Ortholog: 3p21.31 Cellular Component: cell surface; cytoplasm; extracellular region; extracellular space; nucleus; protein complex; secretory granule; specific granule Molecular Function:heparin binding; iron ion binding; protein binding; protein serine/threonine kinase activator activity Biological Process: activation of NF-kappaB transcription factor; antibacterial humoral response; antifungal humoral response; cell redox homeostasis; cellular protein metabolic process; humoral immune response; innate immune response in mucosa; iron assimilation by chelation and transport; negative regulation of apoptosis; negative regulation of ATPase activity; negative regulation of lipopolysaccharide-mediated signaling pathway; negative regulation of viral genome replication; negative regulation of viral reproduction; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of osteoblast differentiation; positive regulation of osteoblast proliferation; positive regulation of toll-like receptor 4 signaling pathway; regulation of cytokine production; regulation of tumor necrosis factor production; retinal homeostasis
NCBI Summary:	This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Antimicrobial, antiviral, antifungal and antiparasitic activity has been found for this protein and its peptides. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
UniProt Code:	P02788
NCBI GenInfo Identifier:	85700158
NCBI Gene ID:	4057
NCBI Accession:	P02788.6
UniProt Secondary Accession:	P02788,O00756, Q16780, Q16785, Q16786, Q16789, Q5DSM0 Q8IU92, A8K9U8, B2MV13, B7Z4X2, E7EQH5,
UniProt Related Accession:	P02788
Molecular Weight:	73,161 Da
NCBI Full Name:	Lactotransferrin
NCBI Synonym Full Names:	lactotransferrin
NCBI Official Symbol:	LTF
NCBI Official Synonym Symbols:	LF; HLF2; GIG12; HEL110
NCBI Protein Information:	lactotransferrin
UniProt Protein Name:	Lactotransferrin
UniProt Synonym Protein Names:	Growth-inhibiting protein 12; Talalactoferrin
Protein Family:	Lactotransferrin
UniProt Gene Name:	LTF
UniProt Entry Name:	TRFL_HUMAN

	Western blot analysis of extracts of various cell lines, using LTF antibody (CAB12902) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat kidney using LTF antibody (CAB12902) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human placenta using LTF antibody (CAB12902) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using LTF antibody (CAB12902) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-LTF Antibody (CAB17931)	Human Lactotransferrin (LTF) ELISA Kit
LTF Antibody (PACO10310)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)