Luciferase Reporter Assay Kit (BN01017)

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SKU:
BN01017
€242

Description

ELISA Kit Technical ManualMSDS

Luciferase Reporter Assay Kit

Firefly luciferase has been used as a sensitive reporter for studying gene regulation and function. Luciferase provides a 1000-fold increase in sensitivity in comparison to the standard chloramphenicol acetyltransferase (CAT) assay. Firefly luciferase catalyzes the oxidative carboxylation of luciferin, a reaction with the highest efficiency of any known bioluminescence reaction. The light emission from the reaction can be recorded using a luminometer. Assay Genie's Luciferase Reporter Assay Kit provides a simple means for detecting luciferase activity in transfected eukaryotic cells. For measurement of expressed luciferase in vitro, luciferase is first extracted from transfected cells through cell lysis. Then CoA, ATP, Mg²and buffer are added to the lysate (Inclusion of CoA yields a nearly constant light emission rather than typical flash Kinetics). The luminescent reaction is then triggered by an injection of luciferin, and the emitted light is recorded by a luminometer. By providing CoA, along with ATP, Mg² in an optimized buffer solution, Assay Genie's Luciferase Reporter Assay Kit ensure maximal sensitivity, consistent light output, as well as convenience and consistency when working with multiple samples.

Key information Description

Product SKU

BN01017

Size

200 assays

Detection Method

Luminescence

Species Reactivity

Mammalian

Applications

This Luciferase Reporter Assay Kit provides a simple means for detecting luciferase activity in transfected eukaryotic cells.

Features and Benefits

The assay ensures maximal sensitivity, consistent light output, as well as accuracy and consistency when working with multiple samples.

Kit Components

  • Substrate A
  • Substrate B
  • Cell Lysis Buffer

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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