Lysyl Oxidase Activity Assay Kit (Fluorometric) (BN01101)

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BN01101
  • Lysyl Oxidase Activity Assay Kit (Fluorometric) (BN01101)
  • Lysyl Oxidase Activity Assay Kit (Fluorometric) (BN01101)
$668.36

Description

ELISA Kit Technical ManualMSDS

Lysyl Oxidase Activity Assay Kit (Fluorometric)

Lysyl oxidase is a cell-secreted amine oxidase that catalyzes the final step of collagen and elastin cross-linking. As a result, lysyl oxidase is required for the biosynthesis of functional extracellular matrices. Inhibition of lysyl oxidase can result in osteolathyrism, a condition characterized by fragile connective tissue and paralysis. Recent work has also suggested lysyl oxidase to play an important role in cancer development: Lysyl oxidase promotes tumor growth and progression in vivo, cancer cell invasion, and premetastatic niche formation. Assay Genie's Lysyl Oxidase Activity Fluorometric Assay Kit allows for quantitative evaluation of lysyl oxidase activity of purified enzyme and its inhibitors as well as secreted endogenous enzyme in cell media. Enzyme activity is detected upon oxidation of substrate, which results in release of reaction intermediates. Reaction intermediates are subsequently detected by Assay Genie's LOX Probe, resulting in fluorescent signal that can be measured at Ex/Em= 535/587 nm. Each kit includes a positive control as well as a specific lysyl oxidase inhibitor that allows the user to correct each sample for any non-specific signal. Lysyl Oxidase Activity Fluorometric Assay Kit can detect as little as 40 ng of recombinant enzyme in vitro.

                                    a)

          b)

Figure: a) Enzyme activity at varying concentrations of recombinant Lysyl Oxidase b) representative activity curve for cell media sample and cell media spike standard at 37°C, where cell media is test sample, cell media + inhibitor is negative control and 150 pmol spike standard is sample + spike standard.

Key Information Description

Product SKU

BN01101

Size

100 Assays

Kit Summary

  • Detection method - Flurometric (535/587 nm)
  • Species reactivity - Mammalians
  • Applications - Measurement of Lysyl Oxidase activity in various biological samples, and purified proteins Includes active lysyl oxidase

Detection Method

Fluorometric (Ex/Em = 535/587 nm)

Species Reactivity

Mammalian

Applications

Measurement of Lysyl Oxidase activity in various biological samples and protein preparations

Features and Benefits

  • Simple, highly sensitive, high-throughput compatible
  • Includes active Lysyl Oxidase

Kit Components

  • LOX Assay Buffer
  • LOX Substrate
  • LOX Developer
  • LOX Probe (in DMSO)
  • LOX Positive Control
  • LOX Inhibitor
  • LOX Standard (0.88 M)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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