Human WWTR1 Recombinant Protein (RPPB5154)
- SKU:
- RPPB5154
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9GZV5
Description
| Product Name: | Human WWTR1 Recombinant Protein |
| Product Code: | RPPB5154 |
| Size: | 20µg |
| Species: | Human |
| Target: | WWTR1 |
| Synonyms: | WW domain-containing transcription regulator protein 1, Transcriptional coactivator with PDZ-binding motif, WWTR1, WW Domain Containing Transcription Regulator 1, TAZ. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The WWTR1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M UREA and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMNPASAP PPLPPPGQQV IHVTQDLDTD LEALFNSVMN PKPSSWRKKI LPESFFKEPD SGSHSRQSST DSSGGHPGPR LAGGAQHVRS HSSPASLQLG TGAGAAGSPA QQHAHLRQQS YDVTDELPLP PGWEMTFTAT GQRYFLNHIE KITTWQDPRK AMNQPLNHMN LHPAVSSTPV PQRSMAVSQP NLVMNHQHQQ QMAPSTLSQQ NHPTQNPPAG LMSMPNALTT QQQQQQKLRL QRIQMERERI RMRQEELMRQ EAALCRQLPM EAETLAPVQA AVNPPTMTPD MRSITNNSSD PFLNGGPYHS REQSTDSGLG LGCYSVPTTP EDFLSNVDEM DTGENAGQTP MNINPQQTRF PDFLDCLPGT NVDLGTLESE DLIPLFNDVE SALNKSEPFL TWL |
WW Domain Containing Transcription Regulator 1 (WWTR1) is a transcriptional coactivator that plays a role as a downstream regulatory target in the Hippo signaling pathway which participates in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. WWTR1 regulates the nuclear accumulation of SMADS and has a main part in coupling them to the transcriptional machinery like the mediator complex. WWTR1 is also regulates embryonic stem-cell self-renewal.
WWTR1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 423 amino acids (1-400) and having a molecular mass of 46.5 kDa.WWTR1 is fused to a 23 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TAZ: Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition. Binds to SLC9A3R2 via the PDZ motif at the plasma membrane. Binds to YWHAZ in vivo and in vitro through the phosphoserine-binding motif RSHSSP. Interacts (via coiled-coil domain) with SMAD2 (via MH1 domain), SMAD3 and SMAD4. Interacts with MED15, PAX8 and NKX2-1. Interacts with TEAD1, TEAD2, TEAD3 and TEAD4. Highly expressed in kidney, heart, placenta and lung. Expressed in the thyroid tissue. |
| UniProt Protein Details: | Protein type:Oncoprotein; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 3q23-q24 Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus Molecular Function:protein binding; transcription coactivator activity Biological Process: negative regulation of protein amino acid phosphorylation; negative regulation of protein kinase activity; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; stem cell division; transcription initiation from RNA polymerase II promoter |
| UniProt Code: | Q9GZV5 |
| NCBI GenInfo Identifier: | 67462080 |
| NCBI Gene ID: | 25937 |
| NCBI Accession: | Q9GZV5.1 |
| UniProt Secondary Accession: | Q9GZV5,Q8N3P2, Q9Y3W6, D3DNH7, |
| UniProt Related Accession: | Q9GZV5 |
| Molecular Weight: | 44,101 Da |
| NCBI Full Name: | WW domain-containing transcription regulator protein 1 |
| NCBI Synonym Full Names: | WW domain containing transcription regulator 1 |
| NCBI Official Symbol: | WWTR1�� |
| NCBI Official Synonym Symbols: | TAZ�� |
| NCBI Protein Information: | WW domain-containing transcription regulator protein 1 |
| UniProt Protein Name: | WW domain-containing transcription regulator protein 1 |
| UniProt Synonym Protein Names: | Transcriptional coactivator with PDZ-binding motif |
| Protein Family: | WW domain-containing transcription regulator protein |
| UniProt Gene Name: | WWTR1�� |
| UniProt Entry Name: | WWTR1_HUMAN |
