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Anti-MBD3 Antibody (CAB2251)

SKU:
CAB2251
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
€139 - €419
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Description

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Antibody Name:Anti-MBD3 Antibody
Antibody SKU:CAB2251
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human MBD3 (NP_001268383.1).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human, Mouse
Positive Samples:Jurkat, K-562
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human MBD3 (NP_001268383.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MERK SPSG KKFR SKPQ LARY LGGS MDLS TFDF RTGK MLMS KMNK SRQR VRYD SSNQ VKGK PDLN TALP VRQT ASIF KQPV TKIT NHPS NKVK SDPQ KAVD QPRQ LFWE KKLS GLNA FDIA EELV KTMD LPKG LQGV GPGC TDET LLSA IASA LHTS TMPI TGQL SAAV EKNP GVWL NTTQ PLCK AFMV TDED IRKQ EELV QQVR KRLE EALM ADML AHVE ELAR DGEA PLDK ACAE DDDE EDEE EEEE EPDP DPE
Gene ID:53615
Uniprot:O95983
Cellular Location:Chromosome, Nucleus
Calculated MW:29kDa/32kDa
Observed MW:36kDa
Synonyms:MBD3
Background:DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. This gene belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain (MBD). The encoded protein is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. Unlike the other family members, the encoded protein is not capable of binding to methylated DNA. The protein mediates the association of metastasis-associated protein 2 with the core histone deacetylase complex. Alternative splicing results in multiple transcript variants of this gene.
UniProt Protein Function:MBD3: methyl-CpG binding (MBD) proteins appear to bind differentially methylated domains (DMDs) of DNA, to recruit repression complexes, and to silence the locus. The methyl CpG binding domain of human MBD3 may not directly bind mCpG but does interact with the NuRD/Mi2 components HDAC1 and MTA2. Plays a role in histone deacetylation and nucleosome remodeling. Increased levels have been reported in lung cancer and glioblastomas and may be a therapeutic target.
UniProt Protein Details:

Protein type:Gene silencing; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nuclear chromatin; nucleoplasm; protein complex

Molecular Function:DNA binding; histone deacetylase activity; methyl-CpG binding; nucleosomal DNA binding; protein binding

Biological Process: ATP-dependent chromatin remodeling; methylation-dependent chromatin silencing; negative regulation of transcription from RNA polymerase II promoter

NCBI Summary:DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. This gene belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain (MBD). The encoded protein is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. Unlike the other family members, the encoded protein is not capable of binding to methylated DNA. The protein mediates the association of metastasis-associated protein 2 with the core histone deacetylase complex. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
UniProt Code:O95983
NCBI GenInfo Identifier:50400820
NCBI Gene ID:53615
NCBI Accession:O95983.1
UniProt Secondary Accession:O95983,Q6PIL9, Q6PJZ9, Q86XF4, A8K4B7, D6W5Z2,
UniProt Related Accession:O95983
Molecular Weight:29,014 Da
NCBI Full Name:Methyl-CpG-binding domain protein 3
NCBI Synonym Full Names:methyl-CpG binding domain protein 3
NCBI Official Symbol:MBD3  
NCBI Protein Information:methyl-CpG-binding domain protein 3
UniProt Protein Name:Methyl-CpG-binding domain protein 3
UniProt Synonym Protein Names:Methyl-CpG-binding protein MBD3
Protein Family:Methyl-CpG-binding domain protein
UniProt Gene Name:MBD3  
UniProt Entry Name:MBD3_HUMAN
Anti-MBD3 Antibody (CAB2251)Western blot analysis of extracts of various cell lines, using MBD3 antibody (CAB2251) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

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