Mitochondrial DNA Isolation Kit (BN00541)

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SKU:
BN00541
€673

Description

ELISA Kit Technical ManualMSDS

Mitochondrial DNA Isolation Kit

Mitochondria are semiautonomous organelles which functions in aging process, apoptosis, anti-HIV drugs, and cancers. Mitochondrial DNA (mtDNA) has a very high mutation rate and the mutations on mtDNA appear to be related to certain diseases such as diabetes, Alzheimer's disease, and muscle disorders. Isolation and quantification of mtDNA are often required to study the relationships between the diseases and mtDNA. The Mitochondrial DNA Extraction Kit provides convenient tools for isolating mtDNA from a variety of cells and tissues in high yield and purity, without contaminations from genomic DNA. The purified mtDNA can be used for a variety of studies such as enzyme manipulations, Southern blotting, cloning, PCR analysis, and amplifications.

Figure: Mitochondrial DNA was isolated from HeLa cells (5 x 106) and rat liver (~50 mg) according to the kit protocol. DNA was measured using Nanodrop at 260nm

Key Information Description

Product SKU

BN00541

Size

50 assays

Detection Method

Mammalian

Applications

Enzyme manipulations, Southern blotting, cloning, PCR analysis, and amplifications.

Features and Benefits

  • Simple procedure; takes less than 2 hours
  • Fast and convenient
  • The kit provides unique formulations of buffers and reagents for isolating a highly enriched mitochondrial fraction.
  • Mitochondrial DNA are then isolated using reagents included in the kit

Kit Components

  • 5X Cytosol Extraction Buffer
  • Mitochondrial Lysis Buffer
  • Enzyme B Mix (lyophilized)
  • TE Buffer

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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