MMP-1 Inhibitor Screening Kit (Fluorometric) (BN01010)

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SKU:
BN01010
  • MMP-1 Inhibitor Screening Kit (Fluorometric) (BN01010)
  • MMP-1 Inhibitor Screening Kit (Fluorometric) (BN01010)
€702

Description

ELISA Kit Technical ManualMSDS

MMP-1 Inhibitor Screening Kit (Fluorometric)

The Matrix metalloproteinase-1 (MMP-1, Interstitial collagenase, fibroblast collagenase) is a member of a multigene family of calcium-dependent, zinc-containing endoproteinases, the matrix metalloproteinases (MMPs). The MMPs are responsible for the degradation of the extracellular matrix (ECM) including collagens, elastins, gelatin, matrix glycoproteins and proteoglycan during normal development and disease processes. MMPs are regulated by hormones, growth factors and cytokines. MMP-1 belongs to the subclass, the collagenases, and along with MMP-8, and MMP-13 are the only members of the MMP family that are capable of degrading the types I, II and III interstitial collagens with high efficiency. These collagens are primarily found in bone, cartilage and skin. In Assay Genie's MMP-1 Inhibitor Screening Kit, MMP-1 hydrolyzes a specific FRET substrate to release a quenched fluorescent group, which can be detected at Ex/Em = 490/520nm. In presence of potent MMP-1 inhibitors the clevage of substrate will be inhibited or stopped. The kit provides a rapid, simple, sensitive, and reliable test suitable as a high throughput screening assay of MMP-1 inhibitors. For comparison of the relative efficacy of test inhibitors, a control inhibitor, GM 6001 (IC = 0.4 nM for MMP-1) is included.

(a)

      (b)

Figure: (a) MMP-1 activity profile at various concentrations of GM 6001 (b) MMP-1 Inhibition by GM 6001

Key Information Description

Product SKU

BN01010

Size

100 assays

Detection Method

Fluorescence (Ex/Em 490/520 nm)

Applications

The kit can be used to screen inhibitors of MMP-1.

Features and Benefits

  • Simple procedure; takes ~ less than 40 minutes
  • Fast and convenient

Kit Components

  • Assay Buffer
  • Substrate
  • MMP-1 Enzyme
  • Inhibitor Control (5 µM GM 6001)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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