Monoacylglycerol Lipase Inhibitor Screening Kit (Fluorometric) (BN00708)

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BN00708
  • Monoacylglycerol Lipase Inhibitor Screening Kit (Fluorometric) (BN00708)
  • Monoacylglycerol Lipase Inhibitor Screening Kit (Fluorometric) (BN00708)
€673

Description

ELISA Kit Technical ManualMSDS

Monoacylglycerol Lipase Inhibitor Screening Kit (Fluorometric)

Monoacylglycerol Lipase (MAGL, E.C. 3.1.1.23) is an important enzyme that regulates endocannabinoid signaling in human physiology. MAGL is a serine hydrolase that generates free fatty acid and glycerol from monoacylglycerol stores. One free fatty acid that is frequently generated by MAGL activity, arachidonic acid, is a precursor for eicosanoids, a family of pro-inflammatory signaling molecules. The glycerol moiety can also serve various purposes as a metabolic building block and energy source. Inhibition or inactivation of MAGL leads to 2-arachidonalglycerol (2-AG) buildup, and this has myriad biological consequences. Desensitization of the endocannabinoid receptors and reduced addiction withdrawal symptoms are one outcome of MAGL inhibition. In addition, the downstream eicosanoid signaling pathway is affected; this pathway plays a role in diverse physiological processes including inflammation, immunity, nociception and blood pressure. As a central node in the regulation of both lipid signaling and energy mobilization, MAGL is of considerable interest as a therapeutic target. Assay Genie's Monoacylglycerol Lipase Inhibitor Screening Kit provides a straightforward method to identify inhibitors of human MAGL. In the assay, human recombinant MAGL is used to cleave an MAGL substrate, resulting in a fluorescence increase that is abrogated by the presence of an inhibitor for this enzyme. Specific inhibitors for this enzyme are of great clinical interest due to the central role for MAGL in lipid metabolism and energy storage.

(a)

(b)

Figure: (a) Reaction kinetics of MAGL Enzyme in the presence and absence of JJKK-048 Control Inhibitor (10 μM). (b) IC 50 determination for MAGL Control Inhibitor JJKK-048 (IC 50 = 75 pM).

Key Information Description

Product SKU

BN00708

Size

100 assays

Kit Summary

  • Detection method - Fluorescence (Ex/Em = 360/460 nm)
  • Applications- Screening for inhibitors of human MAGL enzyme

Detection Method

Fluorometric (Ex/Em = 360/460 nm)

Species Reactivity

Mammalian

Applications

Screening for inhibitors of human MAGL enzyme

Features and Benefits

  • Simple one-step reaction
  • Takes only 1-2 hrs
  • Non-radiometric fluorescent detection HTP adaptable

Kit Components

  • MAGL Assay Buffer
  • MAGL Substrate (200X)
  • MAGL Enzyme (Human Recombinant)
  • MAGL Control Inhibitor (JJKK-048)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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