Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric) (BN00791)

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BN00791
  • Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric) (BN00791)
  • Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric) (BN00791)
  • Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric) (BN00791)
€613

Description

ELISA Kit Technical ManualMSDS

Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric)

Monoacylglycerol Lipase (MAGL, E.C. 3.1.1.23) is an important enzyme for regulation of endocannabinoid signaling in human physiology. MAGL is a serine hydrolase that generates free fatty acid and glycerol from monoacylglycerols such as 2-arachidonoyl glycerol (2-AG), one of the principal endocannabinoids active in the nervous system. One free fatty acid that is released by MAGL activity, arachidonic acid, is a precursor for eicosanoids, a family of pro-inflammatory signaling molecules. The leftover glycerol moiety can also serve various purposes as a metabolic building block and energy source. Inhibition or inactivation of MAGL leads to 2-AG buildup, and this has myriad biological consequences. Antinociceptive effects, anti-inflammatory and neuroprotective effects in Parkinson's and Alzheimer's diseases as well as reduced addiction withdrawal symptoms are some of the outcomes of MAGL inhibition. In addition, the downstream eicosanoid signaling pathway is affected; this pathway plays a role in diverse physiological processes including inflammation, immunity, nociception and blood pressure. As a central node in the regulation of both lipid signaling and energy mobilization, MAGL is of considerable interest as a therapeutic target. Assay Genie's Monoacylglycerol Lipase activity assay kit provides a quick, sensitive and easy way for measuring MAGL activity in various samples. In this assay, a fluorescent substrate is cleaved to generate arachidonic acid and fluorescent metabolite and the increased fluorescence is measured at Ex/Em 360/460 nm. To identify the signal generated specifically by MAGL, a specific inhibitor is included that allows the user to differentiate MAGL activity from other sources of fluorescence. The assay is simple to perform, high-throughput adaptable and can detect as low as 0.1 mU of MAGL activity

Figure: (a) Fluorescent standard curve; (b) Reaction kinetics of Monoacylglycerol Lipase (MAGL) positive control and MAGL activity in rat midbrain lysate (6 µg protein) using appropriate background controls; (c) Monoacylglycerol Lipase specific activity was calculated in rat midbrain and rat intestinal lysates. Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00791

Size

100 assays

Kit Summary

  • Detection method - Fluorescence (Ex/Em = 360/460 nm)
  • Applications- Measurement of monoacylglycerol lipase activity in various tissues/cells.

Detection Method

Fluorometric (Ex/Em = 360/460 nm)

Species Reactivity

Mammalian

Applications

Measurement of monoacylglycerol lipase activity in various tissues/cells.

Features and Benefits

  • Simple one-step reaction
  • Easy Sample preparation; assay only 1-2 hrs
  • Non-radiometric fluorescent detection
  • HTP adaptable

Kit Components

  • MAGL Assay Buffer
  • MAGL Substrate
  • MAGL Positive Control
  • MAGL Selective Inhibitor (200X)
  • Umbelliferone Standard (10 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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